Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)

Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947) is a rabbit monoclonal antibody that is used to detect STAT6 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, Dot Blot. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

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Images

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	ChIP	Dot	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Expected	Tested	Not recommended	Tested
Mouse	Tested	Tested	Not recommended	Expected	Tested	Not recommended	Expected
Rat	Tested	Expected	Not recommended	Expected	Tested	Not recommended	Expected
Synthetic peptide	Not recommended	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).
Species Human	Dilution info 1/1000	Notes p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).
Species Rat	Dilution info 1/1000	Notes p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Associated Products

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Target data

See full target information Stat6 phospho Y641

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and transcription activator 6, Stat6

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22599-78
Purification technique: Affinity purification Protein A
Specificity: STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of STAT6?

Anti-STAT6 (phospho Y641) [EPR22599-78] (ab263947) specifically detects a band for STAT6 (UniProt: P52633) at a molecular weight of 94kDa.

Trusted by the scientific community

Anti-STAT6 (phospho Y641) [EPR22599-78] (ab263947) was first used in a scientific publication in 2019 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR22599-78] also available for your convenience:
ab263947, Carrier free - ab263950, PE - ab305802, APC - ab305803, HRP - ab305804, Alkaline Phosphatase - ab308655, Alexa Fluor® 488 - ab309630, Alexa Fluor® 647 - ab309996, Alexa Fluor® 594 - ab310359, Alexa Fluor® 555 - ab311881, Alexa Fluor® 568 - ab312350, Alexa Fluor® 750 - ab321521

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) Western blot staining using rabbit Anti-STAT6 (phospho Y641) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) The molecular weight observed is consistent with what has been described in the literature (PMID: 24708771).
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Exposure time: 15 seconds
All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) serum starved for 24 hours, whole cell lysate at 10 µg
Lane 2: RAW264.7 serum starved for 24 hours, then treated with 100 ng/ml mIL-4 for 15 minutes, whole cell lysate 10 ug
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa, 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human kidney (panel A), no staining after alkaline phosphatase treatment (panel B. PMID: 8085155, 16181056). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:5000 dilution. Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug
Lane 2: ab263947 IP in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab263947 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 min
All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Predicted band size: 94 kDa
Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) Western blot staining using rabbit Anti-STAT6 (phospho Y641) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) The molecular weight observed is consistent with what has been described in the literature (PMID: 24708771).
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

Exposure time: 37 seconds
All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947) at 1/5000 dilution
Lane 1: 2.4G2 (rat B cell lymphoma B lymphocyte) serum starved for 24 hours, whole cell lysate at 10 µg
Lane 2: 2.4G2 serum starved for 24 hours, then treated with 100 ng/ml rIL-4 for 15 minutes, whole cell lysate 10 ug
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa, 94 kDa
Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:5000 dilution.
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug
Lane 2: ab263947 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab263947 in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Exposure time: 1 min
All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Predicted band size: 94 kDa
Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) Western blot staining using rabbit Anti-STAT6 (phospho Y641) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) This antibody recognizes three isoforms of STAT6, isoform 1 (110 kDa), isoform 2 (75 kDa), isoform 3 (81 kDa), which is consistent with the Uniprot annotation.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

Exposure time: 15 seconds
All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947) at 1/5000 dilution
Lane 1: Daudi (human Burkitts lymphoma lymphoblast) serum starved for 24 hours, whole cell lysate at 10 µg
Lane 2: Daudi serum starved for 24 hours, then treated with 100 ng/ml IL-4 for 15 minutes, whole cell lysate 10 ug
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa, 75 kDa, 81 kDa
Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Dot blot analysis of STAT6 (phospho Y641) peptide (Lane 1 STAT6 non-phospho peptide peptide (Lane 2), labelling STAT 6 (pY641) with purified ab263947 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 26 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat liver (panel A), no staining after alkaline phosphatase treatment (panel B). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red)/Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1/5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse kidney (panel A), no staining after alkaline phosphatase treatment (panel B, PMID: 23155424). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red)/Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (ab263947)
STAT6 (phospho Y641) western blot using anti-STAT6 (phospho Y641) antibody [EPR22599-78] ab263947. Publication image and figure legend from Zeng, B., Wang, D., et al., 2020, Nutrients, PubMed 32911830.

ab263947 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab263947 please see the product overview.
Effect of SPI on JAK1-STAT6 signaling and TGFβ levels in mouse ileum tissue. (A) qRT-PCR analysis of Jak1, Stat6, Tgfb1, and Tgfb2 mRNA expression levels in mouse ileum tissue (normalized against Actb (β-actin); n = 8). (B) Western blot analysis of JAK1, p-JAK1, STAT6, p-STAT6, pIgR, and TGFβ1 protein levels in mouse ileum tissue (n = 6). (C) The statistical analyses of the Western blot results. Results are normalized to β-actin (n = 6). All data are presented as means ± SEM. NS, not significant (p > 0.05); * p < 0.05; ** p < 0.01. Statistical significance was calculated using t-tests.