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Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, IP, WB, Dot and reacts with Mouse, Human, Rat, Synthetic peptide samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (AB263950), expandable thumbnail
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (AB263950), expandable thumbnail
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (AB263950), expandable thumbnail
  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (AB263950), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (AB263950), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PIPChIPWBDotICC/IF
Human
Tested
Tested
Tested
Not recommended
Expected
Expected
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Expected
Expected
Not recommended
Rat
Expected
Tested
Expected
Not recommended
Expected
Expected
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Species
Human
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Species
Rat
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Expected
Expected

Species
Human
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Species
Rat
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Species
Mouse
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and transcription activator 6, Stat6

Recommended products

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, IP, WB, Dot and reacts with Mouse, Human, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22599-78
Purification technique
Affinity purification Protein A
Specificity

STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab263950 is the carrier-free version of Anti-STAT6 (phospho Y641) antibody [EPR22599-78] ab263947.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com