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Anti-Stathmin 1 antibody [EP1573Y] (ab52630) is a rabbit monoclonal antibody that is used to detect Stathmin 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

-Over 40 publications


Images

Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (AB52630), expandable thumbnail
  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (AB52630), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (AB52630), expandable thumbnail
  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (AB52630), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (AB52630), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

For unpurified use at 1/50

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

For unpurified use at 1/50000

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/10000
Notes

For unpurified use at 1/50000

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/10000
Notes

For unpurified use at 1/50000

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

12 products for Alternative Product

Target data

Function

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

Alternative names

Recommended products

Anti-Stathmin 1 antibody [EP1573Y] (ab52630) is a rabbit monoclonal antibody that is used to detect Stathmin 1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

-Over 40 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1573Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Stathmin 1 antibody [EP1573Y] (ab52630) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trusted by the scientific community


Anti-Stathmin 1 [EP1573Y] (ab52630) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists


Anti-Stathmin 1 [EP1573Y] (ab52630) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EP1573Y] also available for your convenience:
ab52630, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Stathmin 1 antibody [EP1573Y] ab199735, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Stathmin 1 antibody [EP1573Y] ab199812, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Stathmin 1 antibody [EP1573Y] ab215094, Carrier free - Anti-Stathmin 1 antibody [EP1573Y] - BSA and Azide free ab221017



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.

Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

Associated diseases and disorders

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    ab52630 (purified) at 1/30 dilution (2ug) immunoprecipitating Stathmin 1 in HeLa whole cell lysates.
    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab52630 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52630 in HeLa whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Predicted band size: 17 kDa

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    All lanes: Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/50000 dilution

    Lane 1: Mouse brain lysates at 15 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa

    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    All lanes: Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/10000 dilution

    All lanes: Human lymph node lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Stathmin 1 with purified ab52630 at 1/200 dilution (3.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    All lanes: Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/500000 dilution

    All lanes: PC12 lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    ab52630, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections.

  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    ab52630 stained HeLa cells

  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail
    Image courtesy of Qifeng Lin by customer review.

    Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Whole cell lysate prepared from human colon cells was loaded at 20μg. The immunoprecipitation step was performed using Protein A/G. ab52630 used at a 1/200 dilution for 12 hours at 4°C. For WB ab52630 used at a 1/10000 dilution.

    All lanes: Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Predicted band size: 17 kDa

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Overlay histogram showing Jurkat cells stained with ab52630 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab52630 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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