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Rabbit Monoclonal Stathmin 1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

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Images

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (AB101690), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] (AB101690), expandable thumbnail
  • Western blot - Anti-Stathmin 1 antibody [SP49] (AB101690), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] (AB101690), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (AB101690), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)WBICC/IF
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Tested
Tested
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

In details: boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed bycooling at RT for 20 min.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat, Chicken, Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Cow, Dog, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Chicken, Cow, Dog, Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

Alternative names

C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19

Recommended products

Rabbit Monoclonal Stathmin 1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP49
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.

Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

Associated diseases and disorders

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.

Product promise

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7 product images

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690)

    Flow cytometry analysis of Jurkat (human acute T cell leukemia) labeling Stathmin 1 with purified ab101690 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] (ab101690)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Stathmin 1 with Purified ab101690 at 1/400 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Western blot - Anti-Stathmin 1 antibody [SP49] (ab101690)

    All lanes: Western blot - Anti-Stathmin 1 antibody [SP49] (ab101690) at 1/25 dilution

    All lanes: lysate prepared from NIH3T3 cells

    Predicted band size: 17 kDa

    Observed band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] (ab101690)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab101690 at 1/500 (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690)

    Flow cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) labeling Stathmin 1 with purified ab101690 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] (ab101690)

    ab101690 at 1/100 dilution staining Stathmin 1 in formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] (ab101690)

    Flow cytometric analysis of rabbit anti-Stathmin 1 (SP49) antibody ab101690 in HeLa cells(green) compared to negative control of rabbit IgG (blue).

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Product protocols

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