JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236223

Anti-Stathmin 1 antibody [SP49] - BSA and Azide free

1

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal Stathmin 1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

ab101690 at 1/100 dilution staining Stathmin 1 in formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab101690).

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab101690 at 1/500 (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular Flow Cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular flow cytometric analysis of rabbit anti-Stathmin 1 (SP49) antibody ab101690 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (AB236223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Stathmin 1 with Purified ab101690 at 1/400 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP49

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB47328

Anti-Stathmin 1 (phospho S16) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S16) antibody (AB47328)

  • 0
  • 0
Primary Antibodies

AB194752

Anti-Stathmin 1 (phospho S25) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S25) antibody (AB194752)

  • 3
  • 1
Primary Antibodies

AB199812

Alexa Fluor® 647 Anti-Stathmin 1 antibody [EP1573Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Stathmin 1 antibody [EP1573Y] (AB199812)

  • 0
  • 0
Primary Antibodies

AB199735

Alexa Fluor® 488 Anti-Stathmin 1 antibody [EP1573Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Stathmin 1 antibody [EP1573Y] (AB199735)

  • 0
  • 0
Primary Antibodies

AB119991

Anti-Stathmin 1 antibody [EP1572]

RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (AB119991)

  • 5
  • 1
Primary Antibodies

AB221017

Anti-Stathmin 1 antibody [EP1573Y] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] - BSA and Azide free (AB221017)

  • 5
  • 1
Primary Antibodies

AB239888

Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888)

  • 0
  • 0
Primary Antibodies

AB215094

Alexa Fluor® 594 Anti-Stathmin 1 antibody [EP1573Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Stathmin 1 antibody [EP1573Y] (AB215094)

  • 0
  • 0
Primary Antibodies

AB194757

Anti-Stathmin 1 (phospho S38) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S38) antibody (AB194757)

  • 0
  • 0
Primary Antibodies

AB52630

Anti-Stathmin 1 antibody [EP1573Y]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (AB52630)

  • 5
  • 5
Primary Antibodies

AB76583

Anti-Stathmin 1 (phospho S63) antibody [EPR1574]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583)

  • 4
  • 1
Primary Antibodies

AB62336

Anti-Stathmin 1 (phospho S24) antibody [EP2124Y]

RabMAb

Western blot - Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (AB62336)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB76315

Anti-STAT3 (phospho Y705) antibody [EP2147Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (AB76315)

  • 4
  • 21
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

  • 5
  • 67
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed bycooling at RT for 20 min.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab236223 is the carrier-free version of ab101690.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.
Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).
See full target information STMN1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 42:444-463.e10 PubMed38428410

2024

Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Radtke,Ekaterina Postovalova,Arina Varlamova,Alexander Bagaev,Maria Sorokina,Olga Kudryashova,Mark Meerson,Margarita Polyakova,Ilia Galkin,Viktor Svekolkin,Sergey Isaev,Daniil Wiebe,Anna Sharun,Alexander Sarachakov,Grigory Perelman,Yaroslav Lozinsky,Ziv Yaniv,Bradley C Lowekamp,Emily Speranza,Li Yao,Stefania Pittaluga,Arthur L Shaffer,Danny Jonigk,James D Phelan,Theresa Davies-Hill,Da Wei Huang,Pavel Ovcharov,Krystle Nomie,Ekaterina Nuzhdina,Nikita Kotlov,Ravshan Ataullakhanov,Nathan Fowler,Michael Kelly,Jagan Muppidi,Jeremy L Davis,Jonathan M Hernandez,Wyndham H Wilson,Elaine S Jaffe,Louis M Staudt,Mark Roschewski,Ronald N Germain
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com