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Rabbit Recombinant Monoclonal Stathmin 1 phospho S63 antibody. Suitable for IHC-P, IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 6 publications.


Images

Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583), expandable thumbnail
  • Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583), expandable thumbnail
  • Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583), expandable thumbnail
  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (AB76583), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytDotWB
Human
Tested
Not recommended
Tested
Not recommended
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2500 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

Alternative names

C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19

Recommended products

Rabbit Recombinant Monoclonal Stathmin 1 phospho S63 antibody. Suitable for IHC-P, IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1574
Purification technique
Affinity purification Protein A
Specificity

This antibody only detects Stathmin 1 phosphorylated on Serine 62. The antibody immunogen shares 86% homology with Stathmin-2, therefore it is possible that the antibody will cross-react with Stathmin-2 when phosphorylated at serine 97. This has not been assessed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.

Biological function summary

Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.

Pathways

The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.

Associated diseases and disorders

The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail

    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Blocking/Diluting buffer and concentration 2% BSA/TBST

    All lanes: Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 15 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates at 15 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

  • Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail

    Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Dot blot analysis of Stathmin 1 (phospho S63) phospho peptide (Lane 1) and Stathmin 1 non-phospho peptide (Lane 2) labeling Stathmin 1 (phospho S63) with ab76583 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail

    Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Stathmin 1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10 μg with ab76583 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10 μg

    Lane 2: ab76583 IP in HeLa treated with Calyculin A whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76583 in HeLa treated with Calyculin A whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    75kDa band could be stathmin/alpha tubulin complex. (PMID:9369201)

    All lanes: Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail
    Image courtesy of Qifeng Lin by customer review.

    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Alkaline phosphatase treatment removes S63 phosphorylation.
    Samples treated with phosphatase were run alongside the normal lysate, and the phosph-S63 signal is not detected after phosphatase treatment, thus suggesting the signal is very specific to the phosphorylated S63.

    Whole cell lysate prepared from a human colon cancer cell line was loaded at 20μg.
    ab76583 used at a 1/1000 dilution.
    Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.

    All lanes: Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Predicted band size: 17 kDa

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583), expandable thumbnail

    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    All lanes: Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/10000 dilution

    Lane 1: HeLa cell lysate, untreated at 10 µg

    Lane 2: HeLa cell lysate treated with Calyculin A at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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