Rabbit Recombinant Monoclonal Stathmin 1 phospho S63 antibody. Carrier free. Suitable for IHC-P, IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Images
![Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--dot-blot-img15735.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888)")
![Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--immunoprecipitation-img164855.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137114.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (AB239888)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Expected | Expected |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).
Alternative names
C1orf215, LAP18, OP18, STMN1, Stathmin, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, Op18, pp19
Recommended products
Rabbit Recombinant Monoclonal Stathmin 1 phospho S63 antibody. Carrier free. Suitable for IHC-P, IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1574
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody only detects Stathmin 1 phosphorylated on Serine 62. The antibody immunogen shares 86% homology with Stathmin-2, therefore it is possible that the antibody will cross-react with Stathmin-2 when phosphorylated at serine 97. This has not been assessed experimentally.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239888 is the carrier-free version of Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Stathmin 1 also known as Oncoprotein 18 (Op18) is a cytoplasmic phosphoprotein weighing approximately 19 kDa. It functions primarily by regulating microtubule dynamics; it destabilizes microtubules and promotes depolymerization. Stathmin 1 achieves this by binding to tubulin heterodimers preventing their polymerization into microtubules. This protein is widely expressed in neurons immune cells and various cancer cell lines indicating its significant role in cellular processes.
- Biological function summary
Stathmin 1 is important in cell cycle regulation and cell signaling. It does not operate as part of a larger protein complex but rather exerts its effects directly on microtubules. By overriding the stabilization of microtubules it ensures proper mitotic spindle function. This action is especially significant during the G2/M transition and mitosis contributing to the precise segregation of chromosomes.
- Pathways
The regulation of microtubule dynamics by Stathmin 1 plays an important role in cell division and neuronal development. It is involved in the PI3K/AKT signaling pathway which ensures cell survival and proliferation. Stathmin 1 interacts with other regulatory proteins such as Tau which stabilizes microtubules offering a balance between dynamic instability and stabilization. This interaction highlights its involvement in cytoskeletal reorganization essential for both cancer progression and neuronal function.
- Associated diseases and disorders
The altered expression of Stathmin 1 is often associated with various cancers including breast and ovarian cancers. Overexpression leads to enhanced tumor growth and metastasis. It also plays a role in neurological disorders by affecting neuronal microtubules making it of particular interest in research on neurodegenerative diseases like Alzheimer's. Its interaction with proteins like Tau in the brain further connects it to these disorders as both are involved in microtubule modulation important for maintaining cell structure and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--dot-blot-img15735.jpg "Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)")
Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)
Dot blot analysis of Stathmin 1 (phospho S63) phospho peptide (Lane 1) and Stathmin 1 non-phospho peptide (Lane 2) labeling Stathmin 1 (phospho S63) with Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583).
![Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--immunoprecipitation-img164855.jpg "Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)")
Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)
This data was developed using Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583, the same antibody clone in a different buffer formulation.
Stathmin 1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10 μg with Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10 μg
Lane 2: abab76583 IP in HeLa treated with Calyculin A whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583 in HeLa treated with Calyculin A whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
75kDa band could be stathmin/alpha tubulin complex. (PMID: 9369201)
All lanes: Immunoprecipitation - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583)
Predicted band size: 17 kDa
Observed band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888), expandable thumbnail](https://content.abcam.com/products/images/stathmin-1-phospho-s63-antibody-epr1574-bsa-and-azide-free-ab239888--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137114.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] - BSA and Azide free (ab239888)
Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stathmin 1 (phospho S63) antibody [EPR1574] ab76583).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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