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AB73478

Anti-Staufen/STAU1 antibody

5

(6 Reviews)

|

(25 Publications)

Rabbit Polyclonal Staufen/STAU1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 25 publications.

View Alternative Names

STAU, STAU1, Double-stranded RNA-binding protein Staufen homolog 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody (AB73478)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody (AB73478)

ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Staufen/STAU1 antibody (AB73478)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Staufen/STAU1 antibody (AB73478)

IHC image of Staufen/STAU1 staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Immunoprecipitation - Anti-Staufen/STAU1 antibody (AB73478)
  • IP

Unknown

Immunoprecipitation - Anti-Staufen/STAU1 antibody (AB73478)

Staufen/STAU1 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5μg of Rabbit polyclonal to Staufen/STAU1 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
Band : 63kDa : Staufen/STAU1; non specific - 75kDa : We are unsure as to the identity of this extra band.

All lanes:

Immunoprecipitation - Anti-Staufen/STAU1 antibody (ab73478)

Predicted band size: 63 kDa

false

Western blot - Anti-Staufen/STAU1 antibody (AB73478)
  • WB

Project

Western blot - Anti-Staufen/STAU1 antibody (AB73478)

All lanes:

Western blot - Anti-Staufen/STAU1 antibody (ab73478) at 1 µg/mL

Lane 1:

SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

Lane 2:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa,95 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Staufen1 (STAU1) also known as Staufen double-stranded RNA binding protein 1 is an RNA-binding protein that plays a significant role in the regulation of mRNA localization stability and translation. It has a mass of approximately 58 kDa and is expressed in a variety of tissues including the brain testis and heart. STAU1 is known for binding to specific secondary structures in mRNA which influences the post-transcriptional gene regulation processes essential for cell function.
Biological function summary

Staufen1 participates in mRNA transport and localization within cells especially neurons. It forms part of a ribonucleoprotein complex which is involved in the movement of mRNA to distinct subcellular sites facilitating localized protein synthesis. This function is critical for the development and maintenance of cellular structures especially in cells with polarized functions such as neurons where precise protein localization is necessary for synaptic function and plasticity.

Pathways

Staufen1 is essential in processes related to mRNA decay and translation regulation. It is actively involved in the nonsense-mediated mRNA decay pathway ensuring the quality control of mRNA transcripts and preventing the synthesis of defective proteins. Additionally STAU1 interacts with proteins such as UPF1 an important player in this mRNA surveillance mechanism highlighting its role in ensuring accurate protein translation and cellular health.

Abnormalities in STAU1 function have been associated with neurodegenerative diseases and muscular disorders. Its interaction with proteins like SMN responsible for motor neuron maintenance links STAU1 to spinal muscular atrophy. Similarly STAU1's role in the regulation of mRNA at synapses connects it to neuronal disorders including some forms of intellectual disability where local protein synthesis at synapses is disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.. (Microbial infection) Plays a role in virus particles production of many viruses including of HIV-1, HERV-K, ebola virus and influenza virus. Acts by interacting with various viral proteins involved in particle budding process.
See full target information STAU1
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:599 PubMed39155279

2024

Noncanonical formation of SNX5 gene-derived circular RNA regulates cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Tung Chen,Hui-Ju Tsai,Chia-Hua Kan,Chung-Pei Ma,Hui-Wen Chen,Ian Yi-Feng Chang,Hsuan Liu,Chih-Ching Wu,Wei-Yun Chu,Ya-Chun Wu,Kai-Ping Chang,Jau-Song Yu,Bertrand Chin-Ming Tan

Genes & development 38:294-307 PubMed38688681

2024

The lncRNA is trafficked to the cytoplasm as a localized mRNA encoding a small peptide in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xiao,Reem Halabi,Chia-Ho Lin,Mohammad Nazim,Kyu-Hyeon Yeom,Douglas L Black

Molecular and cellular biology 44:43-56 PubMed38347726

2024

Staufen1 Represses the FOXA1-Regulated Transcriptome by Destabilizing FOXA1 mRNA in Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine R Pasterczyk,Xiao Ling Li,Ragini Singh,Meira S Zibitt,Corrine Corrina R Hartford,Lorinc Pongor,Lisa M Jenkins,Yue Hu,Patrick X Zhao,Bruna R Muys,Suresh Kumar,Nitin Roper,Mirit I Aladjem,Yves Pommier,Ioannis Grammatikakis,Ashish Lal

Science advances 8:eabo5578 PubMed36170367

2022

The long noncoding RNA scaffolds neuronal granules to maintain nervous system maturity.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Grzejda,Jana Mach,Johanna Aurelia Schweizer,Barbara Hummel,Andrew Mischa Rezansoff,Florian Eggenhofer,Amol Panhale,Maria-Eleni Lalioti,Nina Cabezas Wallscheid,Rolf Backofen,Johannes Felsenberg,Valérie Hilgers

International journal of molecular sciences 23: PubMed35008641

2021

High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Florence Bonnet-Magnaval,Leïla Halidou Diallo,Valérie Brunchault,Nathalie Laugero,Florent Morfoisse,Florian David,Emilie Roussel,Manon Nougue,Audrey Zamora,Emmanuelle Marchaud,Florence Tatin,Anne-Catherine Prats,Barbara Garmy-Susini,Luc DesGroseillers,Eric Lacazette

Adipocyte 10:350-360 PubMed34224297

2021

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Liang,Yi Jiao,Xueli Gong,Hao Gu,Nuerbiye Nuermaimaiti,Xuanyu Meng,Dihui Liu,Yaqun Guan

BMC cancer 21:120 PubMed33541283

2021

Distinct roles for the RNA-binding protein Staufen1 in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen A Marcellus,Tara E Crawford Parks,Shekoufeh Almasi,Bernard J Jasmin

Frontiers in aging neuroscience 12:578719 PubMed33024434

2020

Effects of Embryonic Inflammation and Adolescent Psychosocial Environment on Cognition and Hippocampal Staufen in Middle-Aged Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Fang Wu,Yue-Ming Zhang,He-Hua Ge,Chong-Yang Ren,Zhe-Zhe Zhang,Lei Cao,Fang Wang,Gui-Hai Chen

Journal of leukocyte biology 109:593-603 PubMed32829531

2020

Regulation of RNA degradation pathways during the lipopolysaccharide response in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chi Lai,Alexander James,John Luff,Paul De Souza,Hazel Quek,Uda Ho,Martin F Lavin,Tara L Roberts

Neurochemistry international 139:104808 PubMed32711020

2020

Glutamate-responsive translation of dendritic GSK3β mRNA triggers a cycle for amplification of reactivated preexisting GSK3β that is indispensable for tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Tanaka,Sachiyo Ohashi,Akihiko Takashima,Shunsuke Kobayashi
View all publications
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