Anti-Staufen/STAU1 antibody [EPR7966]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

Immunofluorescent analysis of HepG2 cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

This image was generated using the unpurified version of the product.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

Overlay histogram showing SHSY-5Y cells stained with ab137100 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137100, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the unpurified version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

Immunofluorescent analysis of HeLa cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

This image was generated using the unpurified version of the product.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Staufen/STAU1 with Purified ab137100 at 1/50 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Staufen/STAU1 with Purified ab137100 at 1 : 100 dilution (5.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

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Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

All lanes:

Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 5:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 55 kDa,63 kDa

false

• WB

Lab

Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (AB137100)

All lanes:

Western blot - Anti-Staufen/STAU1 antibody [EPR7966] (ab137100) at 1/1000 dilution

All lanes:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 55 kDa,63 kDa

false