Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (AB240107)

Overlay histogram showing SHSY-5Y cells stained with ab137100 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137100, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137100).

This image was generated using the unpurified version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (AB240107)

Immunofluorescent analysis of HepG2 cells labelling Staufen/STAU1 with ab137100 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137100).

This image was generated using the unpurified version of the product.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (AB240107)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Staufen/STAU1 with Purified ab137100 at 1 : 100 dilution (5.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (AB240107)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Staufen/STAU1 with Purified ab137100 at 1/50 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody [EPR7966] - BSA and Azide free (AB240107)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Staufen/STAU1 with ab137100 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab137100).

This image was generated using the unpurified version of the product.