Rabbit Polyclonal STCH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA13 aa 200-400.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSPA13 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48723
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
Heat shock 70 kDa protein 13, Microsomal stress-70 protein ATPase core, Stress-70 protein chaperone microsome-associated 60 kDa protein, STCH, HSPA13
Recommended products
Rabbit Polyclonal STCH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA13 aa 200-400.
Alternative names
Heat shock 70 kDa protein 13, Microsomal stress-70 protein ATPase core, Stress-70 protein chaperone microsome-associated 60 kDa protein, STCH, HSPA13
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSPA13 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P48723
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STCH also known as Stress-70 protein chaperone is an important molecular chaperone belonging to the Hsp70 family. It weighs approximately 66 kDa and is mainly expressed in the endoplasmic reticulum. STCH helps in protein folding and preventing aggregation which is necessary for maintaining protein homeostasis. This protein's function is less understood compared to other Hsp70 homologs but is believed to be activated under stress conditions like heat shock.
- Biological function summary
STCH participates in managing protein maturation and degradation processes. It works by stabilizing nascent polypeptide chains and assisting in their proper folding though not always forming stable complexes with other proteins. Its localization to the endoplasmic reticulum suggests a specific role in dealing with secretory and membrane proteins. This chaperone guards the cellular proteostasis against stress-induced misfolding a critical process in maintaining normal cellular function.
- Pathways
STCH is involved in the unfolded protein response (UPR) and other stress-related pathways. The UPR plays a role in cellular stress management and signaling mechanisms. STCH interacts with other chaperones like BiP/GRP78 and GRP94 which are significant in these pathways. Through its activity STCH contributes to protein quality control and mitigates proteotoxic stress which affects cellular health and function.
- Associated diseases and disorders
The role of STCH appears in neurodegenerative diseases and cancer. Misregulation of chaperone proteins like STCH can contribute to the progression of Alzheimer's disease through its impact on proteostasis and amyloid-beta peptide folding. In cancer association with proteins such as Hsp90 indicates STCH's influence on tumor growth and survival possibly offering a target for therapeutic intervention. Understanding STCH's function in these contexts remains an active research area.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-STCH antibody (ab127750)
All lanes: Western blot - Anti-STCH antibody (ab127750) at 1/500 dilution
All lanes: U-87 MG whole cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-STCH antibody (ab127750)
ab127750 at 1/500 dilution staining STCH in methanol-fixed Hela cells by Immunofluorescence.
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