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AB290738

Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free

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Rabbit Recombinant Monoclonal STEAP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.

View Alternative Names

PRSS24, STEAP, STEAP1, STEAP1 protein, Six-transmembrane epithelial antigen of prostate 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)

This data was developed using ab290723, the same antibody clone in a different buffer formulation Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling STEAP1 with ab290723 at 1/250 (1.664 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in LNCaP cell lineNegative control : MCF-7 (PMID : 10588738) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)
  • WB

Supplier Data

Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)

This data was developed using ab290723, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody recognizes overexpressed STEAP1B isoform 1 and STEAP1B isoform 2. The band at 250 kDa may be aggregated protein due to boiling. Exposure time : 1 second

All lanes:

Western blot - Anti-STEAP1 antibody [EPR26044-70] (<a href='/en-us/products/primary-antibodies/steap1-antibody-epr26044-70-ab290723'>ab290723</a>) at 1/1000 dilution

Lane 1:

His-tagged human STEAP1 recombinant fragment at 10 ng

Lane 2:

HEK-293T cells transfected with a human STEAP1B (isoform 2) expression vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a human STEAP1B (isoform 1) expression vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 4:

HEK-293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate, at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 14 kDa,32 kDa

false

Exposure time: 1s

Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)
  • WB

Supplier Data

Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)

This data was developed using ab290723, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 16622681). Negative control : MCF7 (PMID : 10588738, 16622681 ) Exposure time : 15 seconds

All lanes:

Western blot - Anti-STEAP1 antibody [EPR26044-70] (<a href='/en-us/products/primary-antibodies/steap1-antibody-epr26044-70-ab290723'>ab290723</a>) at 1/1000 dilution

Lane 1:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 35 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26044-70

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes overexpressed human STEAP1B isoform 1 and isoform 2 (#Q6NZ63). The antibody may also recognise endogenous STEAP1B isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STEAP1 also known as Six Transmembrane Epithelial Antigen of the Prostate 1 is a plasma membrane protein with a molecular weight around 50 kDa. This protein is expressed primarily in prostate tissues but also in a variety of other epithelial tissues. It plays a mechanical role as a metalloreductase facilitating the reduction of metal ions such as iron and copper across the cell membrane. STEAP1 features a characteristic six transmembrane domain which is important for its activity and localization.
Biological function summary

STEAP1 impacts cellular processes connected to metal ion homeostasis and proliferation. By performing as a metalloreductase it modulates the redox state and availability of metal ions within cells. STEAP1 functions as part of a larger complex interacting with other transmembrane proteins in the regulation of ion transport and reduction reactions. These roles are significant in maintaining cellular metabolism and oxidative balance.

Pathways

STEAP1 is actively involved in pathways related to iron and copper metabolism. It links to the iron uptake and transport pathways ensuring proper cellular iron levels are maintained. STEAP1 also shares functional relationships with proteins such as ferritin which stores iron and transferrin which transports iron in the bloodstream. STEAP1 therefore plays an important part in connecting cellular metabolism to systemic metal homeostasis.

STEAP1 has notable associations with prostate cancer and Ewing's sarcoma. Elevated expression of STEAP1 often correlates with the progression of prostate cancer hinting at a potential role in tumor growth and survival. In Ewing's sarcoma STEAP1 engages in molecular mechanisms related to the disease's pathology. Interaction with proteins such as PCA3 a prostate cancer antigen underlines its involvement in carcinogenic processes and highlights its potential as a therapeutic target in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Does not function as a metalloreductase due to the absence of binding sites for the electron-donating substrate NADPH. Promotes Fe(3+) reduction when fused to the NADPH-binding domain of STEAP4.
See full target information STEAP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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