Rabbit Monoclonal STEAP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--western-blot-img403531.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)")
![Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--western-blot-img403530.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)")
![Immunocytochemistry - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--immunocytochemistry-img403529.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (AB290738)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Does not function as a metalloreductase due to the absence of binding sites for the electron-donating substrate NADPH. Promotes Fe(3+) reduction when fused to the NADPH-binding domain of STEAP4.
Alternative names
PRSS24, STEAP, STEAP1, STEAP1 protein, Six-transmembrane epithelial antigen of prostate 1
Recommended products
Rabbit Monoclonal STEAP1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26044-70
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes overexpressed human STEAP1B isoform 1 and isoform 2 (#Q6NZ63). The antibody may also recognise endogenous STEAP1B isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STEAP1 also known as Six Transmembrane Epithelial Antigen of the Prostate 1 is a plasma membrane protein with a molecular weight around 50 kDa. This protein is expressed primarily in prostate tissues but also in a variety of other epithelial tissues. It plays a mechanical role as a metalloreductase facilitating the reduction of metal ions such as iron and copper across the cell membrane. STEAP1 features a characteristic six transmembrane domain which is important for its activity and localization.
- Biological function summary
STEAP1 impacts cellular processes connected to metal ion homeostasis and proliferation. By performing as a metalloreductase it modulates the redox state and availability of metal ions within cells. STEAP1 functions as part of a larger complex interacting with other transmembrane proteins in the regulation of ion transport and reduction reactions. These roles are significant in maintaining cellular metabolism and oxidative balance.
- Pathways
STEAP1 is actively involved in pathways related to iron and copper metabolism. It links to the iron uptake and transport pathways ensuring proper cellular iron levels are maintained. STEAP1 also shares functional relationships with proteins such as ferritin which stores iron and transferrin which transports iron in the bloodstream. STEAP1 therefore plays an important part in connecting cellular metabolism to systemic metal homeostasis.
- Associated diseases and disorders
STEAP1 has notable associations with prostate cancer and Ewing's sarcoma. Elevated expression of STEAP1 often correlates with the progression of prostate cancer hinting at a potential role in tumor growth and survival. In Ewing's sarcoma STEAP1 engages in molecular mechanisms related to the disease's pathology. Interaction with proteins such as PCA3 a prostate cancer antigen underlines its involvement in carcinogenic processes and highlights its potential as a therapeutic target in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--western-blot-img403531.jpg "Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)")
Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)
This data was developed using Anti-STEAP1 antibody [EPR26044-70] ab290723, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 16622681).
Negative control: MCF7 (PMID:10588738, 16622681 )
Exposure time: 15 secondsAll lanes: Western blot - Anti-STEAP1 antibody [EPR26044-70] (Anti-STEAP1 antibody [EPR26044-70] ab290723) at 1/1000 dilution
Lane 1: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 35 kDa
Exposure time: 15s
![Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--western-blot-img403530.jpg "Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)")
Western blot - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)
This data was developed using Anti-STEAP1 antibody [EPR26044-70] ab290723, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody recognizes overexpressed STEAP1B isoform 1 and STEAP1B isoform 2. The band at 250 kDa may be aggregated protein due to boiling.
Exposure time: 1 secondAll lanes: Western blot - Anti-STEAP1 antibody [EPR26044-70] (Anti-STEAP1 antibody [EPR26044-70] ab290723) at 1/1000 dilution
Lane 1: His-tagged human STEAP1 recombinant fragment at 10 ng
Lane 2: HEK-293T cells transfected with a human STEAP1B (isoform 2) expression vector containing a myc-his tag, whole cell lysate at 20 µg
Lane 3: HEK-293T cells transfected with a human STEAP1B (isoform 1) expression vector containing a myc-his tag, whole cell lysate at 20 µg
Lane 4: HEK-293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate, at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 14 kDa, 32 kDa
Exposure time: 1s
![Immunocytochemistry - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738), expandable thumbnail](https://content.abcam.com/products/images/steap1-antibody-epr26044-70-bsa-and-azide-free-ab290738--immunocytochemistry-img403529.jpg "Immunocytochemistry - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)")
Immunocytochemistry - Anti-STEAP1 antibody [EPR26044-70] - BSA and Azide free (ab290738)
This data was developed using Anti-STEAP1 antibody [EPR26044-70] ab290723, the same antibody clone in a different buffer formulation
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling STEAP1 with Anti-STEAP1 antibody [EPR26044-70] ab290723 at 1/250 (1.664 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in LNCaP cell lineNegative control: MCF-7 (PMID: 10588738) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com