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Rabbit Recombinant Monoclonal STEAP1 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.

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Images

Western blot - Anti-STEAP1 antibody [EPR29701-75] (AB324263), expandable thumbnail
  • Western blot - Anti-STEAP1 antibody [EPR29701-75] (AB324263), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (AB324263), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (AB324263), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (AB324263), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Does not function as a metalloreductase due to the absence of binding sites for the electron-donating substrate NADPH. Promotes Fe(3+) reduction when fused to the NADPH-binding domain of STEAP4.

Alternative names

PRSS24, STEAP, STEAP1, STEAP1 protein, Six-transmembrane epithelial antigen of prostate 1

Rabbit Recombinant Monoclonal STEAP1 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29701-75
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Western blot staining using rabbit Anti-STEAP1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human STEAP1B1, STEAP1B2, STEAP2, STEAP3, STEAP4.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human STEAP1 expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human STEAP1B1 expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human STEAP1B2 expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a human STEAP2 expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 6: 293T cells transfected with a human STEAP3 expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 7: 293T cells transfected with a human STEAP4 expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa, 32 kDa, 15-50 kDa, 15 kDa

    Exposure time: 6s

  • Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Western blot staining using rabbit Anti-STEAP1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: PC-3, DU 145 (PMID: 37041154).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 37041154).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-STEAP1 antibody [EPR29701-75] (ab324263) at 1/1000 dilution

    Lane 1: 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30 kDa, 32 kDa, 36 kDa

    Exposure time: 70s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-STEAP1 antibody

    Immunohistochemical analysis of paraffin-embedded (A) LNCaP (human prostate carcinoma epithelial cell) cell pellet. (B) PC-3 (human prostate adenocarcinoma epithelial cell) cell pellet. tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) LNCaP cell pellet, no staining on (B) PC-3 cell pellet.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-STEAP1 antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining on human cerebrum (PMID: 10588738).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica BiosystemsBOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon using rabbit Anti-STEAP1 antibody

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining on human colon (PMID: 10588738).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] (ab324263)

    STEAP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human prostate carcinoma using rabbit Anti-STEAP1 antibody

    Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human prostate carcinoma (PMID: 10588738).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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