Rabbit Recombinant Monoclonal STEAP2 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | Flow Cyt | WB | |
---|---|---|---|
Human | Tested | Not recommended | Not recommended |
Transfected cell line - Human | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (By similarity). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity).
PCANAP1, STAMP1, UNQ6507/PRO23203, STEAP2, Metalloreductase STEAP2, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, PUMPCn
Rabbit Recombinant Monoclonal STEAP2 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human prostate carcinoma using rabbit Anti-STEAP2 antibody
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human prostate carcinoma (PMID: 37966111). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-STEAP2 antibody
Immunohistochemical analysis of paraffin-embedded: (A)LNCap (human prostate carcinoma epithelial cell) cell pellet; (B)PC3 (human prostate adenocarcinoma epithelial cell) cell pellet; (C)Hela (human cervical adenocarcinoma epithelial cell) cell pellet tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive on A: LNCap cell pellet, no staining on (B) PC3 cell pellet and (C) Hela cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-STEAP2 antibody
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human STEAP2 expression vector containing a His tag. (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag. (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag. (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag. (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag. (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag. (G) HEK-293T transfected with empty vector containing a His tag. tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a human STEAP2 expression vector containing a His tag, no staining on (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag, (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag, (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag, (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag, (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag, and (G) HEK-293T transfected with empty vector containing a His tag. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-STEAP2 antibody
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human kidney (PMID: 37966111). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-STEAP2 antibody
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling STEAP2 with ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human skeletal muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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