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Rabbit Recombinant Monoclonal STEAP2 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (AB324357), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line - Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (By similarity). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity).

Alternative names

PCANAP1, STAMP1, UNQ6507/PRO23203, STEAP2, Metalloreductase STEAP2, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, PUMPCn

Rabbit Recombinant Monoclonal STEAP2 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR30542-566
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab324357 is the carrier free version of Anti-STEAP2 antibody [EPR30542-566] ab324346.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357)

    STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human prostate carcinoma using rabbit Anti-STEAP2 antibody

    This data was developed using Anti-STEAP2 antibody [EPR30542-566] ab324346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with Anti-STEAP2 antibody [EPR30542-566] ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human prostate carcinoma (PMID: 37966111). The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357)

    STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-STEAP2 antibody

    This data was developed using Anti-STEAP2 antibody [EPR30542-566] ab324346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded: (A)LNCap (human prostate carcinoma epithelial cell) cell pellet; (B)PC3 (human prostate adenocarcinoma epithelial cell) cell pellet; (C)Hela (human cervical adenocarcinoma epithelial cell) cell pellet tissue labeling STEAP2 with Anti-STEAP2 antibody [EPR30542-566] ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive on A: LNCap cell pellet, no staining on (B) PC3 cell pellet and (C) Hela cell pellet. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357)

    STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-STEAP2 antibody

    This data was developed using Anti-STEAP2 antibody [EPR30542-566] ab324346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human STEAP2 expression vector containing a His tag. (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag. (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag. (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag. (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag. (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag. (G) HEK-293T transfected with empty vector containing a His tag. tissue labeling STEAP2 with Anti-STEAP2 antibody [EPR30542-566] ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a human STEAP2 expression vector containing a His tag, no staining on (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag, (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag, (D) HEK-293T transfected with a human STEAP1 expression vector containing a His tag, (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag, (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag, and (G) HEK-293T transfected with empty vector containing a His tag. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357)

    STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-STEAP2 antibody

    This data was developed using Anti-STEAP2 antibody [EPR30542-566] ab324346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STEAP2 with Anti-STEAP2 antibody [EPR30542-566] ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human kidney (PMID: 37966111). The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody [EPR30542-566] - BSA and Azide free (ab324357)

    STEAP2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human skeletal muscle using rabbit Anti-STEAP2 antibody

    This data was developed using Anti-STEAP2 antibody [EPR30542-566] ab324346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling STEAP2 with Anti-STEAP2 antibody [EPR30542-566] ab324346 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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