JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126068

Anti-STEAP3 antibody

5

(1 Review)

|

(1 Publication)

Rabbit Polyclonal STEAP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STEAP3 aa 1-250.

View Alternative Names

TSAP6, STEAP3, Metalloreductase STEAP3, Dudulin-2, Six-transmembrane epithelial antigen of prostate 3, Tumor suppressor-activated pathway protein 6, pHyde, hTSAP6, hpHyde

1 Images
Western blot - Anti-STEAP3 antibody (AB126068)
  • WB

Unknown

Western blot - Anti-STEAP3 antibody (AB126068)

10% SDS PAGE

All lanes:

Western blot - Anti-STEAP3 antibody (ab126068) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human STEAP3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q658P3

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STEAP3 also known as Six-Transmembrane Epithelial Antigen of Prostate 3 or TSAP6 acts as a metalloreductase. It is involved in the reduction of metal ions particularly iron. The protein facilitates the conversion of ferric to ferrous iron which is essential for cellular uptake of iron through divalent metal transporters. STEAP3 with a molecular weight of approximately 50 kDa is mainly expressed in prostate tissue but also found in other tissues like the liver and testis.
Biological function summary

STEAP3 plays an important role in iron homeostasis by contributing to iron metabolism and mobilization. The protein represents a component of the multiprotein complex in endosomes where it participates alongside proteins such as ferrireductase and divalent metal transporter 1 (DMT1). These interactions are important for maintaining proper cellular iron levels and preventing iron deficiency or overload.

Pathways

Iron metabolism and recycling are heavily influenced by the activities of STEAP3. The protein is important within the endosomal ferrireductase pathway where it collaborates closely with DMT1 and transferrin receptor proteins to mediate iron uptake and release. Additionally STEAP3 participates in the heme biosynthesis pathway indirectly by ensuring adequate availability of iron which is central to heme production and function.

Researchers have observed associations between STEAP3 dysregulation and conditions like anemia and cancer. Deficient STEAP3 activity can contribute to iron-related disorders such as anemia linked via its relation to the transferrin receptor which mediates iron uptake in cells. Conversely overexpression of STEAP3 has implications in cancer potentially linked to its interactions with p53 a well-known tumor suppressor protein indicating a role in cancer cell iron metabolism and growth.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (PubMed : 26205815). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity). Mediates efficient transferrin-dependent iron uptake in erythroid cells (By similarity). May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression (By similarity). Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP (PubMed : 15319436, PubMed : 16651434).
See full target information STEAP3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 14:4119-4123 PubMed27667392

2016

Dysregulation of GTPase IMAP family members in hepatocellular cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Huang,Wei Zhang,Chunhai Gao,Baoju Ji,Xiuwen Chi,Wei Zheng,Hai Lan Wang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Primary Antibodies

AB214039

Anti-Transferrin Receptor antibody [EPR20584]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] (AB214039)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB55735

Anti-DMT1 antibody [4C6]

KO Validated

Western blot - Anti-DMT1 antibody [4C6] (AB55735)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com