Rabbit Recombinant Monoclonal STEAP3 antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.
Images
![Western blot - Anti-STEAP3 antibody [EPR9812] - BSA and Azide free (AB249009), expandable thumbnail](https://content.abcam.com/products/images/steap3-antibody-epr9812-bsa-and-azide-free-ab249009--western-blot-img105126.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STEAP3 antibody [EPR9812] - BSA and Azide free (AB249009)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Target data
Function
Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (PubMed:26205815). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity). Mediates efficient transferrin-dependent iron uptake in erythroid cells (By similarity). May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression (By similarity). Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP (PubMed:15319436, PubMed:16651434).
Alternative names
TSAP6, STEAP3, Metalloreductase STEAP3, Dudulin-2, Six-transmembrane epithelial antigen of prostate 3, Tumor suppressor-activated pathway protein 6, pHyde, hTSAP6, hpHyde
Recommended products
Rabbit Recombinant Monoclonal STEAP3 antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9812
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249009 is the carrier-free version of Anti-STEAP3 antibody [EPR9812] ab151566.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STEAP3 also known as Six-Transmembrane Epithelial Antigen of Prostate 3 or TSAP6 acts as a metalloreductase. It is involved in the reduction of metal ions particularly iron. The protein facilitates the conversion of ferric to ferrous iron which is essential for cellular uptake of iron through divalent metal transporters. STEAP3 with a molecular weight of approximately 50 kDa is mainly expressed in prostate tissue but also found in other tissues like the liver and testis.
- Biological function summary
STEAP3 plays an important role in iron homeostasis by contributing to iron metabolism and mobilization. The protein represents a component of the multiprotein complex in endosomes where it participates alongside proteins such as ferrireductase and divalent metal transporter 1 (DMT1). These interactions are important for maintaining proper cellular iron levels and preventing iron deficiency or overload.
- Pathways
Iron metabolism and recycling are heavily influenced by the activities of STEAP3. The protein is important within the endosomal ferrireductase pathway where it collaborates closely with DMT1 and transferrin receptor proteins to mediate iron uptake and release. Additionally STEAP3 participates in the heme biosynthesis pathway indirectly by ensuring adequate availability of iron which is central to heme production and function.
- Associated diseases and disorders
Researchers have observed associations between STEAP3 dysregulation and conditions like anemia and cancer. Deficient STEAP3 activity can contribute to iron-related disorders such as anemia linked via its relation to the transferrin receptor which mediates iron uptake in cells. Conversely overexpression of STEAP3 has implications in cancer potentially linked to its interactions with p53 a well-known tumor suppressor protein indicating a role in cancer cell iron metabolism and growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-STEAP3 antibody [EPR9812] - BSA and Azide free (ab249009), expandable thumbnail](https://content.abcam.com/products/images/steap3-antibody-epr9812-bsa-and-azide-free-ab249009--western-blot-img105126.jpg "Western blot - Anti-STEAP3 antibody [EPR9812] - BSA and Azide free (ab249009)")
Western blot - Anti-STEAP3 antibody [EPR9812] - BSA and Azide free (ab249009)
This data was developed using Anti-STEAP3 antibody [EPR9812] ab151566, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-STEAP3 antibody [EPR9812] (Anti-STEAP3 antibody [EPR9812] ab151566) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: PC12 cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Downloads
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- Visit the general protocols
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