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AB74531

Anti-STELLAR antibody

1

(1 Review)

|

(2 Publications)

Rabbit Polyclonal STELLAR antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DPPA3.

View Alternative Names

STELLAR, DPPA3, Developmental pluripotency-associated protein 3, Stella-related protein

1 Images
Western blot - Anti-STELLAR antibody (AB74531)
  • WB

Unknown

Western blot - Anti-STELLAR antibody (AB74531)

Lane 1:

Western blot - Anti-STELLAR antibody (ab74531) at 1 µg/mL

Lane 2:

Western blot - Anti-STELLAR antibody (ab74531) at 2 µg/mL

All lanes:

293 cell lysate at 15 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DPPA3. The exact immunogen used to generate this antibody is proprietary information.

Q6W0C5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STELLAR also known as 'STELLA' or 'DPPA2' acts mechanically as a regulator of pluripotency and early embryonic development. It weighs approximately 17 kDa. This protein expresses itself in pluripotent stem cells and germ cells. Researchers have found subcellular localization of STELLAR mainly in the nucleus suggesting critical functions in gene regulation during cell differentiation.
Biological function summary

The protein plays an important role in maintaining cell totipotency and germ cell specification. STELLAR interacts with several cellular complexes that are pivotal during embryogenesis. By regulating specific genes STELLAR contributes to the repression of inappropriate gene expression in pluripotent cells impacting cellular fate outcomes during development.

Pathways

STELLAR participates in the pluripotency regulatory network involving significant pathways like the Wnt signaling pathway. This protein's regulatory mechanisms align with other transcription factors such as NANOG and OCT4 highlighting its role in the maintenance of the pluripotent state. The interplay among these factors establishes a complex circuit essential for the retention of stem cell properties.

Aberrant expression or regulation of STELLAR associates with certain cancers. Its connection to disorders like germ cell tumors suggests an oncogenic potential when dysregulated. Moreover STELLAR's interaction with proteins such as LIN28 highlights its involvement in tumorigenic processes shedding light on its significance in both normal development and pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed : 35314832). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process (By similarity). Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC : specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation (By similarity). Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark (By similarity). Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis (By similarity). May be important for the totipotent/pluripotent states continuing through preimplantation development (By similarity). Also involved in chromatin condensation in oocytogenesis (By similarity).
See full target information DPPA3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reviews and reports 19:1067-1081 PubMed36735215

2023

Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Yu,Ning Wang,Xiang Wang,Hehe Ren,Yanping Zhang,Yingxin Zhang,Yikai Qiu,Hongyan Wang,Guoping Wang,Xiuying Pei,Ping Chen,Yahui Ren,Chunfang Ha,Li Wang,Huayan Wang

International journal of molecular medicine 35:1219-29 PubMed25760093

2015

Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Hu,Hua Lu,Sheng Cao,Yan-Li Deng,Qi-Jia Li,Qian Wan,Shang-Mian Yie
View all publications
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Product promise

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