AB313479

Anti-STELLAR Antibody [RP23040195]

Recombinant
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Rabbit Recombinant Monoclonal STELLAR antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DPPA3.

View Alternative Names

STELLAR, DPPA3, Developmental pluripotency-associated protein 3, Stella-related protein

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-STELLAR Antibody [RP23040195] (AB313479)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STELLAR Antibody [RP23040195] (AB313479)

Immunofluorscent analysis of NCCIT cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous DPPa3 with ab313479 at 2 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of DPPa3 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of DPPa3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RP23040195

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DPPA3.

Q6W0C5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STELLAR also known as 'STELLA' or 'DPPA2' acts mechanically as a regulator of pluripotency and early embryonic development. It weighs approximately 17 kDa. This protein expresses itself in pluripotent stem cells and germ cells. Researchers have found subcellular localization of STELLAR mainly in the nucleus suggesting critical functions in gene regulation during cell differentiation.

Biological function summary

The protein plays an important role in maintaining cell totipotency and germ cell specification. STELLAR interacts with several cellular complexes that are pivotal during embryogenesis. By regulating specific genes STELLAR contributes to the repression of inappropriate gene expression in pluripotent cells impacting cellular fate outcomes during development.

Pathways

STELLAR participates in the pluripotency regulatory network involving significant pathways like the Wnt signaling pathway. This protein's regulatory mechanisms align with other transcription factors such as NANOG and OCT4 highlighting its role in the maintenance of the pluripotent state. The interplay among these factors establishes a complex circuit essential for the retention of stem cell properties.

Aberrant expression or regulation of STELLAR associates with certain cancers. Its connection to disorders like germ cell tumors suggests an oncogenic potential when dysregulated. Moreover STELLAR's interaction with proteins such as LIN28 highlights its involvement in tumorigenic processes shedding light on its significance in both normal development and pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed : 35314832). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process (By similarity). Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC : specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation (By similarity). Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark (By similarity). Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis (By similarity). May be important for the totipotent/pluripotent states continuing through preimplantation development (By similarity). Also involved in chromatin condensation in oocytogenesis (By similarity).

See full target information DPPA3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-STELLAR Antibody [RP23040195]

Immunocytochemistry/ Immunofluorescence - Anti-STELLAR Antibody [RP23040195] (AB313479)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise