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AB155329

Anti-STEP / PTPN5 antibody

1

(1 Review)

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(1 Publication)

Rabbit Polyclonal STEP / PTPN5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PTPN5 aa 300 to C-terminus.

View Alternative Names

Tyrosine-protein phosphatase non-receptor type 5, Neural-specific protein-tyrosine phosphatase, Striatum-enriched protein-tyrosine phosphatase, STEP, PTPN5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEP / PTPN5 antibody (AB155329)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEP / PTPN5 antibody (AB155329)

Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling STEP/PTPN5 with ab155329 at 1/500 dilution.

Western blot - Anti-STEP / PTPN5 antibody (AB155329)
  • WB

Unknown

Western blot - Anti-STEP / PTPN5 antibody (AB155329)

7.5% SDS PAGE

All lanes:

Western blot - Anti-STEP / PTPN5 antibody (ab155329) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 64 kDa

false

Western blot - Anti-STEP / PTPN5 antibody (AB155329)
  • WB

Unknown

Western blot - Anti-STEP / PTPN5 antibody (AB155329)

7.5% SDS PAGE

All lanes:

Western blot - Anti-STEP / PTPN5 antibody (ab155329) at 1/1000 dilution

All lanes:

mouse brain lysate at 50 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PTPN5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54829

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Tris/EDTA buffer (pH 8.0) may also be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STEP also known as PTPN5 is a protein tyrosine phosphatase with a molecular mass approximately 69-80 kDa. It regulates the activity of key signaling proteins by dephosphorylation. Expression of STEP occurs mainly in the central nervous system particularly in the striatum hippocampus and cortex regions of the brain. These areas of expression suggest its significant role in neuronal signaling and function. STEP exists in multiple isoforms which allow it to participate in various signaling pathways by altering substrate specificity.
Biological function summary

STEP plays a significant role in modulating synaptic strength and plasticity acting as a negative regulator of synaptic signaling. Its main function involves dephosphorylating several kinase substrates like ERK1/2 and Fyn kinase thereby reducing their activity. This action controls synaptic sensitivity to neurotransmitters and impacts long-term potentiation a process essential for learning and memory. STEP does not form part of a large complex but interacts with other proteins through transient associations based on cellular needs.

Pathways

STEP contributes significantly to the glutamatergic and dopaminergic signaling pathways. Within these pathways STEP dephosphorylates and inactivates specific receptors and kinases to maintain homeostasis in neuronal signaling. For example in the glutamatergic pathway STEP deactivates NMDA receptors affecting calcium influx and signaling cascades that rely on it. Its interactions with other proteins like ERK which is part of the MAPK pathway illustrate its integrated role in signal transduction cascades that influence cellular responses to neurotransmitters.

STEP has a relationship with neurodegenerative diseases such as Alzheimer's disease and schizophrenia. Increased activity of STEP leads to excessive dephosphorylation of proteins necessary for synaptic function contributing to the cognitive deficits observed in these disorders. In Alzheimer's disease for instance hyperactive STEP decreases the phosphorylation of the NMDA receptor and disrupts synaptic transmission. Additionally STEP's influence extends to dynamin-related protein 1 (Drp1) in neurodegenerative pathways indicating how altered STEP activity can escalate mitochondrial dysfunction commonly seen in Alzheimer's pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
See full target information PTPN5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 20:250 PubMed32994813

2020

TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Weng,Song Zheng,Hanli Shui,Guosheng Lin,Yongjian Zhou
View all publications
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Product promise

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