Anti-steroidogenic Factor 1/SF-1 antibody [EPR19744] is a rabbit monoclonal antibody used to detect steroidogenic factor 1 (SF-1) in immunohistochemistry on paraffin-embedded sections (IHC-P), chromatin immunocleavage (ChIC) and CUT&RUN sequencing. Suitable for human samples.
- Recombinant format ensures high batch-to-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65237.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124806.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76414.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)")
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439406.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)")
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439405.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)")
Publications
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- Pituitary 26:227-2362023TGFBR3L is associated with gonadotropin production in non-functioning gonadotroph pituitary neuroendocrine tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnders Jensen Kolnes,Kristin Astrid Berland Øystese,Evelina Sjöstedt,Nicoleta Cristina Olarescu,Ansgar Heck,Jens Pahnke,Daniel Dahlberg,Jon Berg-Johnsen,Geir Ringstad,Olivera Casar-Borota,Jens Bollerslev,Anders Palmstrøm JørgensenPubMed 36952069
- Frontiers in cell and developmental biology 9:6663032021Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Tan,Lingling Zhang,Tianqi Li,Jianmin Zhan,Kun Qiao,Haili Wu,Shenfei Sun,Meina Huang,Fangxi Zhang,Meixing Zhang,Changwei Li,Runsheng Li,Hongjie PanPubMed 34631693
- Cancers 13:2020TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvelina Sjöstedt et. al.PubMed 33396509
- The Journal of clinical endocrinology and metabolism 106:1183-11942020Corticotroph Aggressive Pituitary Tumors and Carcinomas Frequently Harbor ATRX Mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivera Casar-Borota,Henning Bünsow Boldt,Britt Edén Engström,Marianne Skovsager Andersen,Bertrand Baussart,Daniel Bengtsson,Katarina Berinder,Bertil Ekman,Ulla Feldt-Rasmussen,Charlotte Höybye,Jens Otto L Jørgensen,Anders Jensen Kolnes,Márta Korbonits,Åse Krogh Rasmussen,John R Lindsay,Paul Benjamin Loughrey,Dominique Maiter,Emilija Manojlovic-Gacic,Jens Pahnke,Pietro Luigi Poliani,Vera Popovic,Oskar Ragnarsson,Camilla Schalin-Jäntti,David Scheie,Miklós Tóth,Chiara Villa,Martin Wirenfeldt,Jacek Kunicki,Pia BurmanPubMed 33106857
- The Journal of clinical endocrinology and metabolism 105:2020Pituitary Gangliocytoma Producing TSH and TRH: A Review of "Gangliocytomas of the Sellar Region".Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyohiko Sakata,Kana Fujimori,Satoru Komaki,Takuya Furuta,Yasuo Sugita,Kenji Ashida,Masatoshi Nomura,Motohiro MoriokaPubMed 32706866
- Cancer cell 37:123-134.e52019Pangenomic Classification of Pituitary Neuroendocrine Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Neou,Chiara Villa,Roberta Armignacco,Anne Jouinot,Marie-Laure Raffin-Sanson,Amandine Septier,Franck Letourneur,Ségolène Diry,Marc Diedisheim,Brigitte Izac,Cassandra Gaspar,Karine Perlemoine,Victoria Verjus,Michèle Bernier,Anne Boulin,Jean-François Emile,Xavier Bertagna,Florence Jaffrezic,Denis Laloe,Bertrand Baussart,Jérôme Bertherat,Stephan Gaillard,Guillaume AssiéPubMed 31883967
- Cancers 11:2019How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Eugenia Torregrosa-Quesada et. al.PubMed 31835737
- Molecular and cellular endocrinology 498:1105852019In vitro cytotoxicity of cabazitaxel in adrenocortical carcinoma cell lines and human adrenocortical carcinoma primary cell cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Fragni,Lilian Patricia Palma Lopez,Elisa Rossini,Andrea Abate,Deborah Cosentini,Valentina Salvi,Sara Vezzoli,Pietro Luigi Poliani,Daniela Bosisio,Constanze Hantel,Guido A M Tiberio,Salvatore Grisanti,Maurizio Memo,Massimo Terzolo,Alfredo Berruti,Sandra SigalaPubMed 31536779
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ChIC/CUT&RUN-seq | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg | Notes - |
Target data
Function
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed:27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed:27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Alternative names
AD4BP, FTZF1, SF1, NR5A1, Steroidogenic factor 1, SF-1, STF-1, hSF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP
Recommended products
Anti-steroidogenic Factor 1/SF-1 antibody [EPR19744] is a rabbit monoclonal antibody used to detect steroidogenic factor 1 (SF-1) in immunohistochemistry on paraffin-embedded sections (IHC-P), chromatin immunocleavage (ChIC) and CUT&RUN sequencing. Suitable for human samples.
- Recombinant format ensures high batch-to-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19744
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65237.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human ovary is observed [PMID: 20660055] [PMID: 10370224]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124806.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human testis is observed [PMID: 10370224]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76414.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
Immunohistochemical analysis of paraffin-embedded human adrenal cancer tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human adrenal cancer is observed [PMID: 20660055]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439406.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439405.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439404.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-ab217317--chic-cut-run-sequencing-img439404.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (ab217317)")
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