Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

Immunohistochemical analysis of paraffin-embedded human adrenal cancer tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human adrenal cancer is observed [PMID : 20660055]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217317).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human testis is observed [PMID : 10370224]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217317).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Steroidogenic Factor 1/SF-1 with ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human ovary is observed [PMID : 20660055] [PMID : 10370224]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217317).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

This data was developed using the same antibody clone in a different buffer formulation (ab217317).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

This data was developed using the same antibody clone in a different buffer formulation (ab217317).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)

This data was developed using the same antibody clone in a different buffer formulation (ab217317).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.