Rabbit Recombinant Monoclonal Steroidogenic Factor 1/SF-1 antibody. Carrier free. Suitable for IHC-P, ChIC/CUT&RUN-seq and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65237.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124806.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76414.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)")
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439406.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)")
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439405.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (AB240394)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ChIC/CUT&RUN-seq | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed:27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed:27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Alternative names
AD4BP, FTZF1, SF1, NR5A1, Steroidogenic factor 1, SF-1, STF-1, hSF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP
Recommended products
Rabbit Recombinant Monoclonal Steroidogenic Factor 1/SF-1 antibody. Carrier free. Suitable for IHC-P, ChIC/CUT&RUN-seq and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19744
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240394 is the carrier-free version of Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65237.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Steroidogenic Factor 1/SF-1 with Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human ovary is observed [PMID: 20660055] [PMID: 10370224]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124806.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Steroidogenic Factor 1/SF-1 with Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human testis is observed [PMID: 10370224]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76414.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
Immunohistochemical analysis of paraffin-embedded human adrenal cancer tissue labeling Steroidogenic Factor 1/SF-1 with Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human adrenal cancer is observed [PMID: 20660055]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439406.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439405.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439404.jpg "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 HepG2 (human hepatocellular carcinoma epithelial cell) cells and 5 µg of Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] ab217317 [EPR19744]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394), expandable thumbnail](https://content.abcam.com/products/images/steroidogenic-factor-1-sf-1-antibody-epr19744-bsa-and-azide-free-ab240394--chic-cut-run-sequencing-img439404.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] - BSA and Azide free (ab240394)")
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