JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB222838

Anti-STIL/SIL antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal STIL/SIL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STIL aa 350 to C-terminus.

View Alternative Names

SIL, STIL, SCL-interrupting locus protein, TAL-1-interrupting locus protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (AB222838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (AB222838)

Paraffin-embedded human breast cancer tissue stained for STIL/SIL using ab222838 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-STIL/SIL antibody (AB222838)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STIL/SIL antibody (AB222838)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for STIL/SIL (green) using ab222838 at 1/100 dilution in ICC/IF. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) was used as secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (AB222838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (AB222838)

Paraffin-embedded human lung cancer tissue stained for STIL/SIL using ab222838 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human STIL aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15468

style
left-column

Alternative Products

Primary Antibodies

AB89314

Anti-STIL/SIL antibody

Immunoprecipitation - Anti-STIL/SIL antibody (AB89314)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB315222

Anti-PLK4 antibody [EPR27156-51]

20ul selling size|RabMAb|Recombinant|Trial Size

Western blot - Anti-PLK4 antibody [EPR27156-51] (AB315222)

  • 0
  • 0
Primary Antibodies

AB219831

Anti-CEP350 antibody [CL3423]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP350 antibody [CL3423] (AB219831)

  • 5
  • 1
Primary Antibodies

AB228891

Anti-SDCCAG8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SDCCAG8 antibody (AB228891)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STIL also known as SIL (SCL/TAL1 Interrupting Locus) is a protein involved mechanically in cellular processes that are important for cell division. STIL has a molecular mass of approximately 150 kDa. This protein localizes primarily in the nucleus where it is abundantly expressed in actively dividing cells. It plays a direct role in maintaining proper cell cycle progression especially during mitosis.
Biological function summary

The STIL protein plays a role in regulating centrosome duplication ensuring accurate chromosome segregation. STIL forms part of a complex with other proteins like SAS-6 which is important in forming the mitotic spindle apparatus. This action is essential to maintaining the structural integrity of centrosomes which are key for correct cell division.

Pathways

The STIL protein integrates into cell cycle regulation and the centrosome cycle. STIL interacts with proteins such as PLK4 and CEP135 within the centrosome duplication pathway. This interaction is important for the initiation of procentriole biogenesis aligning with the G1 phase of the cell cycle.

Misregulation of the STIL protein associates strongly with cancer and microcephaly. For instance overexpression of STIL links to various cancers as it can lead to abnormal cell proliferation. Additionally mutations in the STIL gene are connected to microcephaly a condition characterized by underdeveloped cerebral cortex. Here STIL mutation affects proteins like MCPH1 which is involved in brain development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1 (PubMed : 16024801, PubMed : 9372240). Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CPAP to the base of the procentriole to initiate procentriole assembly (PubMed : 22020124). In complex with STIL acts as a modulator of PLK4-driven cytoskeletal rearrangements and directional cell motility (PubMed : 29712910, PubMed : 32107292).
See full target information STIL
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed33491649

2021

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre Gönczy

Gene 686:220-227 PubMed30453068

2018

The human oncogene SCL/TAL1 interrupting locus (STIL) promotes tumor growth through MAPK/ERK, PI3K/Akt and AMPK pathways in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xingcheng Wu,Yu Xiao,Weigang Yan,Zhigang Ji,Guoyang Zheng
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com