Rabbit Polyclonal STIL/SIL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STIL aa 350-650.
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- eLife 10:2021TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre GönczyPubMed 33491649
- Gene 686:220-2272018The human oncogene SCL/TAL1 interrupting locus (STIL) promotes tumor growth through MAPK/ERK, PI3K/Akt and AMPK pathways in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingcheng Wu,Yu Xiao,Weigang Yan,Zhigang Ji,Guoyang ZhengPubMed 30453068
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human STIL aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q15468
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
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Target data
Function
Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1 (PubMed:16024801, PubMed:9372240). Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly (PubMed:22020124). In complex with STIL acts as a modulator of PLK4-driven cytoskeletal rearrangements and directional cell motility (PubMed:29712910, PubMed:32107292).
Alternative names
SIL, STIL, SCL-interrupting locus protein, TAL-1-interrupting locus protein
Recommended products
Rabbit Polyclonal STIL/SIL antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STIL aa 350-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human STIL aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q15468
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
>95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STIL also known as SIL (SCL/TAL1 Interrupting Locus) is a protein involved mechanically in cellular processes that are important for cell division. STIL has a molecular mass of approximately 150 kDa. This protein localizes primarily in the nucleus where it is abundantly expressed in actively dividing cells. It plays a direct role in maintaining proper cell cycle progression especially during mitosis.
- Biological function summary
The STIL protein plays a role in regulating centrosome duplication ensuring accurate chromosome segregation. STIL forms part of a complex with other proteins like SAS-6 which is important in forming the mitotic spindle apparatus. This action is essential to maintaining the structural integrity of centrosomes which are key for correct cell division.
- Pathways
The STIL protein integrates into cell cycle regulation and the centrosome cycle. STIL interacts with proteins such as PLK4 and CEP135 within the centrosome duplication pathway. This interaction is important for the initiation of procentriole biogenesis aligning with the G1 phase of the cell cycle.
- Associated diseases and disorders
Misregulation of the STIL protein associates strongly with cancer and microcephaly. For instance overexpression of STIL links to various cancers as it can lead to abnormal cell proliferation. Additionally mutations in the STIL gene are connected to microcephaly a condition characterized by underdeveloped cerebral cortex. Here STIL mutation affects proteins like MCPH1 which is involved in brain development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-STIL/SIL antibody (ab222838)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for STIL/SIL (green) using ab222838 at 1/100 dilution in ICC/IF. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (ab222838)
Paraffin-embedded human breast cancer tissue stained for STIL/SIL using ab222838 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIL/SIL antibody (ab222838)
Paraffin-embedded human lung cancer tissue stained for STIL/SIL using ab222838 at 1/100 dilution in immunohistochemical analysis.
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