Rabbit Polyclonal STIL/SIL antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human STIL aa 1200 to C-terminus.
View Alternative Names
SIL, STIL, SCL-interrupting locus protein, TAL-1-interrupting locus protein
- IP
Unknown
Immunoprecipitation - Anti-STIL/SIL antibody (AB89314)
Detection of STIL/SIL in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab89314 at 3 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with exposure time of 30 seconds.
All lanes:
Immunoprecipitation - Anti-STIL/SIL antibody (ab89314)
Predicted band size: 143 kDa
false
- WB
Supplier Data
Western blot - Anti-STIL/SIL antibody (AB89314)
All lanes:
Western blot - Anti-STIL/SIL antibody (ab89314) at 0.1 µg/mL
Lane 1:
TCMK-1 cell lysate at 50 µg
Lane 2:
4T1 cell lysate at 50 µg
Lane 3:
CT26.WT cell lysate at 50 µg
Predicted band size: 143 kDa
false
Exposure time: 3min
- WB
CiteAb
Western blot - Anti-STIL/SIL antibody (AB89314)
STIL/SIL western blot using anti-STIL/SIL antibody ab89314. Publication image and figure legend from Ohta, M., Ashikawa, T., et al., 2014, Nat Commun, PubMed 25342035.
ab89314 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab89314 please see the product overview.
Direct interaction of Plk4 with STIL is required for centriolar targeting of STIL and centriole formation.(a) Co-immunoprecipitation (co-IP) assays testing interactions between the indicated Plk4-FLAG proteins and endogenous STIL. HEK293T cells expressing the empty FLAG vector (–), FLAG-tagged Plk4 full-length (FL) wild-type (FL-WT), FL kinase dead (FL-KD), ΔPEST (Δ272–311 a.a.) WT (ΔP-WT) or ΔPEST KD (ΔP-KD) were immunoprecipitated (IP) with FLAG antibodies. Total cell lysates and IPs were analysed by western blot using STIL, HsSAS-6, FLAG or tubulin antibodies. (b) Schematic of HA-tagged STIL FL and deletion constructs used for co-IP assays with Plk4ΔPEST-FLAG in HEK293T cells. The right columns show a summary of the co-IP results and centriolar localization of the STIL constructs examined in U2OS cells. The STIL constructs that interact with Plk4ΔPEST-FLAG are represented in red and the minimal binding region in light green. The evolutionarily conserved coiled-coil (CC : a.a. 721–746) and STAN (a.a. 1,061–1,147) domains are indicated. ND, not determined. (c) Alignment of the CC domain within human, mouse, Xenopus and zebrafish STIL and Drosophila Ana2. Identical residues are coloured in yellow; similar residues in grey. Asterisks indicate the residues identical in all aligned sequences; colons : conserved substitutions; periods : semi-conserved substitutions. (d) HEK293T cells co-expressing Plk4ΔPEST-FLAG and HA-STIL FL or STILΔCC were IP with FLAG antibodies. Total cell lysates and IPs were analysed by western blot using the indicated antibodies. (e) Yeast two-hybrid assay testing interactions between FL or the indicated fragment (a.a. 661–1,017) of STIL and WT or KD Plk4. The empty vectors (–) were used for negative controls. Two independent clones were grown on the plates without histidine and containing 50 mM 3-AT (Supplementary Fig. 1g). (f) U2OS cells were treated with control siRNA (siCnt) or siRNA targeting 3'UTR of endogenous STIL (siSTIL), followed by transfection with an empty vector (vec; –), HA-STIL FL, N-terminal fragment (N), ΔCC or C-terminal fragment (C). The cells were immunostained with antibodies against HA and centrin. DNA is shown in blue. Insets show approximately fivefold magnified views around the centrosome. Scale bar, 5 μm. Histograms represent frequency of interphase cells with centriolar HA (top) or with ≥4 centrin foci (bottom) in each condition. Values are mean percentages±s.d. from three independent experiments (N>50 for each condition). *P<0.05, **P<0.01, NS, not significant (one-tailed t-test).
false
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Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The STIL protein plays a role in regulating centrosome duplication ensuring accurate chromosome segregation. STIL forms part of a complex with other proteins like SAS-6 which is important in forming the mitotic spindle apparatus. This action is essential to maintaining the structural integrity of centrosomes which are key for correct cell division.
Pathways
The STIL protein integrates into cell cycle regulation and the centrosome cycle. STIL interacts with proteins such as PLK4 and CEP135 within the centrosome duplication pathway. This interaction is important for the initiation of procentriole biogenesis aligning with the G1 phase of the cell cycle.
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Target data
Publications (32)
Recent publications for all applications. Explore the full list and refine your search
eLife 13: PubMed40067174
2025
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Cell division 20:1 PubMed39825314
2025
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Oncology research 33:123-132 PubMed39735672
2024
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Cell death and differentiation 30:2151-2166 PubMed37596441
2023
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JACS Au 3:2247-2256 PubMed37654580
2023
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Nature communications 14:1522 PubMed36934096
2023
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The Journal of biological chemistry 298:102658 PubMed36356903
2022
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The Journal of cell biology 220: PubMed33351100
2020
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Current biology : CB 30:2395-2403.e4 PubMed32442461
2020
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Molecules and cells 42:840-849 PubMed31722512
2019
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