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AB241510

Anti-STIM2 antibody

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(1 Publication)

Rabbit Polyclonal STIM2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STIM2 aa 600-650.

View Alternative Names

KIAA1482, STIM2, Stromal interaction molecule 2

2 Images
Immunoprecipitation - Anti-STIM2 antibody (AB241510)
  • IP

Supplier Data

Immunoprecipitation - Anti-STIM2 antibody (AB241510)

STIM2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241510 used for IP at 6 μg per reaction. Western blot was perfomed on the immunoprecipitate using another anti-STIM2 antibody at 1 μg/ml.

Lane 1 : ab241510 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-STIM2 antibody (ab241510)

Predicted band size: 84 kDa

false

Western blot - Anti-STIM2 antibody (AB241510)
  • WB

Supplier Data

Western blot - Anti-STIM2 antibody (AB241510)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-STIM2 antibody (ab241510) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 84 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human STIM2 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

Q9P246

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241510 was affinity purified using an epitope specific to STIM2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STIM2 also known as Stromal Interaction Molecule 2 is a protein involved in calcium signaling with a mass of approximately 82 kDa. It is expressed in various tissues including the brain where it functions to regulate calcium entry into cells. STIM2 acts as a sensor that detects decreases in intracellular calcium levels and helps mediate the opening of calcium channels in the plasma membrane.
Biological function summary

The STIM2 protein facilitates calcium homeostasis by working in cooperation with other proteins to form complexes. It plays an essential role in cellular processes by maintaining basal calcium levels. In neuronal cells STIM2 helps regulate synaptic activity and plasticity impacting learning and memory functions. The protein's ability to form complexes and modulate calcium stores is critical for effective cellular signaling.

Pathways

STIM2 is a fundamental component of the store-operated calcium entry (SOCE) pathway which maintains cellular calcium levels. It functions alongside its homolog STIM1 to activate calcium release-activated calcium channels such as ORAI1 facilitating calcium influx into the cell. This regulation is important for the immune responses and muscle function where precise calcium signaling is necessary for optimal cellular performance.

STIM2 has been implicated in neurological disorders such as Alzheimer's disease where altered calcium signaling can contribute to disease progression. STIM2 dysregulation may affect synaptic stability and memory formation leading to cognitive impairment. Additionally the protein associates with disorders involving immune system dysfunction where abnormal SOCE activity plays a role. Its interaction with STIM1 and ORAI1 in these conditions underlines the importance of balanced calcium homeostasis for proper cellular function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
See full target information STIM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:733-743 PubMed39380528

2024

Mechanism of KMT2D-mediated epigenetic modification in IL-1β-induced nucleus pulposus cell degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjiang Liu,Haiquan Liu,Zuyu Meng,Wensheng Zhang
View all publications
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