Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)

Rabbit Recombinant Monoclonal STING antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-STING antibody [EPR25090-107] - BSA and Azide free (AB288164), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Expected	Tested	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information Sting1

Function

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:18818105, PubMed:19433799, PubMed:19776740, PubMed:26229117, PubMed:26669264, PubMed:27324217, PubMed:28529930, PubMed:29973723, PubMed:31346090). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (PubMed:18818105, PubMed:19433799, PubMed:19776740, PubMed:26229117, PubMed:26669264). Acts by binding cyclic dinucleotides: recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (PubMed:21947006, PubMed:23258412, PubMed:23519410, PubMed:23722158, PubMed:23910378). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state (PubMed:25636800, PubMed:27324217, PubMed:29973723). Exhibits 2',3' phosphodiester linkage-specific ligand recognition: can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed:26300263). The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (By similarity). In addition to promote the production of type I interferons, plays a direct role in autophagy (PubMed:30568238). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (By similarity). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (By similarity). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (By similarity). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (By similarity). Autophagy is also triggered upon infection by bacteria: following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (PubMed:29056340). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (By similarity). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (PubMed:18559423).

Alternative names

Eris, Mita, Mpys, Sting, Tmem173, Sting1, Stimulator of interferon genes protein, mSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, ERIS, MMITA

Recommended products

Rabbit Recombinant Monoclonal STING antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25090-107
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab288164 is the carrier-free version of Anti-STING antibody [EPR25090-107] ab288157.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The stimulator of interferon genes (STING) also known as TMEM173 or MPYS is a critical transmembrane protein with a molecular weight of approximately 42 kDa. It is primarily expressed in the endoplasmic reticulum of various cell types including immune cells where it plays a central role in sensing cytosolic DNA. STING binds to cyclic dinucleotides produced by the enzyme cGAS upon recognition of aberrant DNA in the cytosol. This binding initiates activation and translocation of STING to the Golgi apparatus facilitating further signaling events.

Biological function summary

STING serves as a pivotal regulator in the innate immune response to viral and bacterial infections. It operates by forming a signaling complex with kinases and other effector proteins which subsequently leads to the activation of transcription factors such as IRF3 and NF-kB. These transcription factors then induce the expression of type I interferons and other cytokines important for mounting an effective antiviral response. The STING pathway therefore enhances the immune system's ability to detect and respond to pathogens.

Pathways

The activity of STING is integral to the cGAS-STING pathway a significant cytosolic DNA-sensing pathway involved in innate immunity. Upon activation STING interacts with TBK1 a kinase that further phosphorylates IRF3 promoting its nuclear translocation and activation. Beyond this STING also intersects with pathways involving autophagy a cellular process necessary for clearing pathogens and damaged cellular components. Through these pathways STING critically contributes to upholding cellular homeostasis and immune defense.

Associated diseases and disorders

The dysregulation of STING is linked to autoinflammatory diseases and certain cancers. Abnormal STING activation can lead to chronic inflammation a feature observed in diseases such as STING-associated vasculopathy with onset in infancy (SAVI). STING's role in cancer is also notable where its ability to activate immune cells can be harnessed in immunotherapy yet its chronic activation may promote tumorigenesis. In cancer STING often interacts with proteins like K-Ras influencing tumor growth and response to therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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18 product images

Immunoprecipitation - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
STING was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with Anti-STING antibody [EPR25090-107] ab288157 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-STING antibody [EPR25090-107] ab288157 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug
Lane 2: Anti-STING antibody [EPR25090-107] ab288157 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-STING antibody [EPR25090-107] ab288157 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
All lanes: Immunoprecipitation - Anti-STING antibody [EPR25090-107] (Anti-STING antibody [EPR25090-107] ab288157)
Predicted band size: 42 kDa
Observed band size: 33 kDa, 33.35 kDa, 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Positive staining on mouse breast cancer. The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 81 seconds
All lanes: Western blot - Anti-STING antibody [EPR25090-107] (Anti-STING antibody [EPR25090-107] ab288157) at 1/1000 dilution
Lane 1: Rat spleen tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 40 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 33 kDa, 35 kDa
Immunohistochemistry (Frozen sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen (fresh) tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/500 (1.114 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labelling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds
All lanes: Western blot - Anti-STING antibody [EPR25090-107] (Anti-STING antibody [EPR25090-107] ab288157) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 33 kDa, 33.35 kDa, 35 kDa
Immunohistochemistry (Frozen sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/500 (1.114 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Western blot - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates/proteins at 20 μg per lane.
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-STING antibody [EPR25090-107] (Anti-STING antibody [EPR25090-107] ab288157) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: J774A.1 (mouse reticum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 4: A20 (mouse reticum sarcoma b lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 33.35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive cytoplasmic staining in RAW 264.7 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 cells labelling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive cytoplasmic staining in J774A.1 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Positive staining on kupffer cells in rat liver (PMID 30561388). The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used. Positive staining on rat spleen. The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Positive staining on kupffer cells in mouse liver (PMID 30561388). The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1/200000) (36KDa)
Blocking buffer and concentration: 5% NFDM/TBST

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Sting1-KO homozygous mice (Strain ID: T012747).
Lanes 1 - 6: Western blot - Anti-STING antibody [EPR25090-107] (Anti-STING antibody [EPR25090-107] ab288157) at 1/1000 dilution
Lanes 1 - 6: Western blot - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
Lane 1: Wild-type mouse spleen tissue lysate (male)
Lanes 2 - 3: STING knockout spleen tissue lysate (male)
Lane 4: Wild-type mouse liver tissue lysate (male)
Lanes 5 - 6: STING knockout liver tissue lysate (male)
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from STING knockout mice labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/5000 followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from STING knockout mice.
The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Sting1-KO homozygous mice (Strain ID: T012747).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR25090-107] - BSA and Azide free (ab288164)
This data was developed using Anti-STING antibody [EPR25090-107] ab288157, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from STING knockout mice labeling STING with Anti-STING antibody [EPR25090-107] ab288157 at 1/5000 followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from STING knockout mice.
The section was incubated with Anti-STING antibody [EPR25090-107] ab288157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Sting1-KO homozygous mice (Strain ID: T012747).