AB227704

Anti-STING antibody [SP338]

BOND RX™ Validated
20ul selling size
KO Validated
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal STING antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.

View Alternative Names

ERIS, MITA, STING, TMEM173, STING1, Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, hMITA

19 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human spleen tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227704 at 1 : 100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227704 for 10 mins at room temperature.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [SP338] (AB227704)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [SP338] (AB227704)

Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling TMEM173 with purified ab227704 at 1/50 (2.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-STING antibody [SP338] (AB227704)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STING antibody [SP338] (AB227704)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227704 at 1/400 dilution (green) compared with a rabbit IgG negative control (blue).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-STING antibody [SP338] (AB227704)

Flow cytometry analysis of THP-1 (human monocytic leukemia monocyte) labeling TMEM173 with purified ab227704 at 1/20 dilution (5.25 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227704 for 10 mins at room temperature.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] (AB227704)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227704 for 10 mins at room temperature.

Lab

Western blot - Anti-STING antibody [SP338] (AB227704)

ab227704 was shown to react with STING1 in wild-type THP-1 cells in Western blot with loss of signal observed in STING1 knockout cell line ab270493. Wild-type THP-1 and STING1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab227704 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-STING antibody [SP338] (ab227704) at 1/200 dilution

Lane 1:

Wild-type THP-1 lysate at 40 µg

Lane 2:

STING1 knock-out THP-1 lysate at 40 µg

false

Supplier Data

Western blot - Anti-STING antibody [SP338] (AB227704)

All lanes:

Western blot - Anti-STING antibody [SP338] (ab227704) at 1/400 dilution

All lanes:

THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP338

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/400", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The stimulator of interferon genes (STING) also known as TMEM173 or MPYS is a critical transmembrane protein with a molecular weight of approximately 42 kDa. It is primarily expressed in the endoplasmic reticulum of various cell types including immune cells where it plays a central role in sensing cytosolic DNA. STING binds to cyclic dinucleotides produced by the enzyme cGAS upon recognition of aberrant DNA in the cytosol. This binding initiates activation and translocation of STING to the Golgi apparatus facilitating further signaling events.

Biological function summary

STING serves as a pivotal regulator in the innate immune response to viral and bacterial infections. It operates by forming a signaling complex with kinases and other effector proteins which subsequently leads to the activation of transcription factors such as IRF3 and NF-kB. These transcription factors then induce the expression of type I interferons and other cytokines important for mounting an effective antiviral response. The STING pathway therefore enhances the immune system's ability to detect and respond to pathogens.

Pathways

The activity of STING is integral to the cGAS-STING pathway a significant cytosolic DNA-sensing pathway involved in innate immunity. Upon activation STING interacts with TBK1 a kinase that further phosphorylates IRF3 promoting its nuclear translocation and activation. Beyond this STING also intersects with pathways involving autophagy a cellular process necessary for clearing pathogens and damaged cellular components. Through these pathways STING critically contributes to upholding cellular homeostasis and immune defense.

The dysregulation of STING is linked to autoinflammatory diseases and certain cancers. Abnormal STING activation can lead to chronic inflammation a feature observed in diseases such as STING-associated vasculopathy with onset in infancy (SAVI). STING's role in cancer is also notable where its ability to activate immune cells can be harnessed in immunotherapy yet its chronic activation may promote tumorigenesis. In cancer STING often interacts with proteins like K-Ras influencing tumor growth and response to therapies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 18724357, PubMed : 18818105, PubMed : 19433799, PubMed : 19776740, PubMed : 23027953, PubMed : 23747010, PubMed : 23910378, PubMed : 27801882, PubMed : 29973723, PubMed : 30842659, PubMed : 35045565, PubMed : 35388221, PubMed : 36808561, PubMed : 37832545). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (PubMed : 26300263). Acts by binding cyclic dinucleotides : recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (PubMed : 21947006, PubMed : 23258412, PubMed : 23707065, PubMed : 23722158, PubMed : 23747010, PubMed : 23910378, PubMed : 26229117, PubMed : 30842659, PubMed : 35388221, PubMed : 37379839). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state (PubMed : 22394562, PubMed : 25636800, PubMed : 29973723, PubMed : 30842653, PubMed : 35045565, PubMed : 35388221). Exhibits 2',3' phosphodiester linkage-specific ligand recognition : can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed : 23747010, PubMed : 23910378, PubMed : 26300263). The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (PubMed : 26150511). In addition to promote the production of type I interferons, plays a direct role in autophagy (PubMed : 30568238, PubMed : 30842662). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (PubMed : 30842662). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (PubMed : 30842662). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (PubMed : 37535724). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (PubMed : 30568238, PubMed : 30842662). Autophagy is also triggered upon infection by bacteria : following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (By similarity). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (PubMed : 18724357). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (By similarity).. (Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed : 26405230). Such oncoproteins prevent the ability to sense cytosolic DNA (PubMed : 26405230).

See full target information STING1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Immune network 25:e25 PubMed40917788

2025

Pathotype-Specific Expression of Granzyme-Perforin Pathway Genes and Their Association With Clinical Disease Activity in Early Rheumatoid Arthritis and in a Randomized Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco G La Rosa,Larry W Moreland,Luigi Nibali,Mike Curtis,Kevin D Deane,Colin Strickland,Jennifer Seifert,Carson Keeter,Dmitri Simberg,Robert I Scheinman,Rachel Lau,Costantino Pitzalis,Myles J Lewis,V Michael Holers,Nirmal K Banda

F1000Research 13:1049 PubMed40535171

2025

A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Ruíz Moleón,Charles Alende,Maryam Fotouhi,Riham Ayoubi,Kathleen Southern,Carl Laflamme

British journal of cancer 129:1852-1862 PubMed37838813

2023

Preoperative chemoradiotherapy induces multiple pathways related to anti-tumour immunity in rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis M Koukourakis,Erasmia Xanthopoulou,Theologos I Sgouras,Maria Kouroupi,Alexandra Giatromanolaki,Vassilios Kouloulias,Dina Tiniakos,Anna Zygogianni

Science advances 7:eabf9441 PubMed34910513

2021

MRE11-dependent instability in mitochondrial DNA fork protection activates a cGAS immune signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica W Luzwick,Eszter Dombi,Rebecca A Boisvert,Sunetra Roy,Soyoung Park,Selvi Kunnimalaiyaan,Steffi Goffart,Detlev Schindler,Katharina Schlacher

International journal of ophthalmology 14:1660-1665 PubMed34804854

2021

Increased cGAS/STING signaling components in patients with Mooren's ulcer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ni Zhang,Yan-Ling Dong,Wen-Pei Hao,Xiao-Fei Bai,Xia Qi,Ting Liu,Xiao-Tong Sun,Chao Wei,Xiao-Lin Qi

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB198952

Alexa Fluor® 647 Anti-STING antibody [EPR13130]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-STING antibody [EPR13130] (AB198952)

Primary Antibodies

AB198951

HRP Anti-STING antibody [EPR13130]

RabMAb|Recombinant

Western blot - HRP Anti-STING antibody [EPR13130] (AB198951)

Primary Antibodies

AB208874

PE Anti-STING antibody [EPR13130]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-STING antibody [EPR13130] (AB208874)

Primary Antibodies

AB181125

Anti-STING antibody [EPR13130]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130] (AB181125)

Primary Antibodies

AB242019

Anti-STING antibody [EPR13130-55] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130-55] - BSA and Azide free (AB242019)

Primary Antibodies

AB239074

Anti-STING antibody [EPR13130-55]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130-55] (AB239074)

Complementary Products

Assay Kits

AB315058

Human STING ELISA Kit

SimpleStep

Sandwich ELISA - Human STING ELISA Kit (AB315058)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB109272

Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (AB109272)

Primary Antibodies

AB76493

Anti-IRF3 (phospho S386) antibody [EPR2346]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (AB76493)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com