Anti-STING antibody [SP338] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human spleen tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling TMEM173 with purified ab227704 at 1/50 (2.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab227704, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Intracellular flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227704 at 1/400 dilution (green) compared with a rabbit IgG negative control (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Intracellular Flow Cytometry analysis of THP-1 (human monocytic leukemia monocyte) labeling TMEM173 with purified ab227704 at 1/20 dilution (5.25μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^®488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

This image was generated using ab227704, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227704).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.

The section was incubated with ab227704 for 10 mins at room temperature.

This image was generated using ab227704, the same clone, but with a different buffer formulation.

• WB

Lab

Western blot - Anti-STING antibody [SP338] - BSA and Azide free (AB238795)

This data was developed using ab227704, the same antibody clone in a different buffer formulation.

ab227704 was shown to react with STING1 in wild-type THP-1 cells in Western blot with loss of signal observed in STING1 knockout cell line ab270493. Wild-type THP-1 and STING1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab227704 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-STING antibody [SP338] (<a href='/en-us/products/primary-antibodies/sting-antibody-sp338-ab227704'>ab227704</a>) at 1/200 dilution

Lane 1:

Wild-type THP-1 lysate at 40 µg

Lane 2:

STING1 knock-out THP-1 lysate at 40 µg

false