Rabbit Recombinant Monoclonal STING phospho S366 antibody. Suitable for WB, Dot and reacts with Synthetic peptide - Human, Human, Mouse samples.
Images
![Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--dot-blot-img446721.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229)")
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446718.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229)")
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446717.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229)")
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446716.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229)")
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446715.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (AB324229)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended |
| Synthetic peptide - Human | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:18724357, PubMed:18818105, PubMed:19433799, PubMed:19776740, PubMed:23027953, PubMed:23747010, PubMed:23910378, PubMed:27801882, PubMed:29973723, PubMed:30842659, PubMed:35045565, PubMed:35388221, PubMed:36808561, PubMed:37832545). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm (PubMed:26300263). Acts by binding cyclic dinucleotides: recognizes and binds cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, cyclic UMP-AMP (2',3'-cUAMP), and cyclic GMP-AMP (cGAMP), a messenger produced by CGAS in response to DNA virus in the cytosol (PubMed:21947006, PubMed:23258412, PubMed:23707065, PubMed:23722158, PubMed:23747010, PubMed:23910378, PubMed:26229117, PubMed:30842659, PubMed:35388221, PubMed:37379839). Upon binding to c-di-GMP, cUAMP or cGAMP, STING1 oligomerizes, translocates from the endoplasmic reticulum and is phosphorylated by TBK1 on the pLxIS motif, leading to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent anti-viral state (PubMed:22394562, PubMed:25636800, PubMed:29973723, PubMed:30842653, PubMed:35045565, PubMed:35388221). Exhibits 2',3' phosphodiester linkage-specific ligand recognition: can bind both 2'-3' linked cGAMP (2'-3'-cGAMP) and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed:23747010, PubMed:23910378, PubMed:26300263). The preference for 2'-3'-cGAMP, compared to other linkage isomers is probably due to the ligand itself, whichs adopts an organized free-ligand conformation that resembles the STING1-bound conformation and pays low energy costs in changing into the active conformation (PubMed:26150511). In addition to promote the production of type I interferons, plays a direct role in autophagy (PubMed:30568238, PubMed:30842662). Following cGAMP-binding, STING1 buds from the endoplasmic reticulum into COPII vesicles, which then form the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) (PubMed:30842662). The ERGIC serves as the membrane source for WIPI2 recruitment and LC3 lipidation, leading to formation of autophagosomes that target cytosolic DNA or DNA viruses for degradation by the lysosome (PubMed:30842662). Promotes autophagy by acting as a proton channel that directs proton efflux from the Golgi to facilitate MAP1LC3B/LC3B lipidation (PubMed:37535724). The autophagy- and interferon-inducing activities can be uncoupled and autophagy induction is independent of TBK1 phosphorylation (PubMed:30568238, PubMed:30842662). Autophagy is also triggered upon infection by bacteria: following c-di-GMP-binding, which is produced by live Gram-positive bacteria, promotes reticulophagy (By similarity). May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons (PubMed:18724357). May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) (By similarity). (Microbial infection) Antiviral activity is antagonized by oncoproteins, such as papillomavirus (HPV) protein E7 and adenovirus early E1A protein (PubMed:26405230). Such oncoproteins prevent the ability to sense cytosolic DNA (PubMed:26405230).
Alternative names
ERIS, MITA, STING, TMEM173, Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, hMITA
Rabbit Recombinant Monoclonal STING phospho S366 antibody. Suitable for WB, Dot and reacts with Synthetic peptide - Human, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR29040-93
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--dot-blot-img446721.jpg "Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)")
Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)
STING (phospho S366) Dot Blot staining using rabbit Anti-STING (phospho S366) antibody
Dot blot analysis of STING (phospho S366) using ab324229 at 1:1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: STING phospho S366 peptide a
Lane2: STING phospho S366 peptide b
Lane3: STING non-phospho peptideExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229) at 1/1000 dilution
Lane 1: STING phospho S366 peptide a
Lane 2: STING phospho S366 peptide b
Lane 3: STING non-phospho peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446718.jpg "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)")
Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)
STING (phospho S366) Western blot staining using rabbit Anti-STING (phospho S366) antibody
Blocking and diluting buffer and concentration: 1% BSA/TBST.
Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) (1/10000) (124KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) (1/5000);
In Western blot, Anti-STING antibody [EPR13130-55] (Anti-STING antibody [EPR13130-55] ab239074) staining at 1/1000 dilution.
All lanes: Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human wild-type STING expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human wild-type STING expression vector containing a myc-His-tag® treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human STING (S366A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 5: 293T cells transfected with a human STING (S366A mutation) expression vector containing a myc-His-tag® treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa, 124 kDa
Exposure time: 59s
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446717.jpg "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)")
Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)
STING (phospho S366) Western blot staining using rabbit Anti-STING (phospho S366) antibody
Blocking and diluting buffer and concentration: 1% BSA/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: RAW 264.7 treated first with 80nM TPA for 24 hours, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa, 124 kDa
Exposure time: 180s
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446716.jpg "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)")
Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)
STING (phospho S366) Western blot staining using rabbit Anti-STING (phospho S366) antibody
Blocking and diluting buffer and concentration: 1% BSA/TBST.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-STING antibody [EPR13130-55] (Anti-STING antibody [EPR13130-55] ab239074) staining at 1/1000 dilution.
All lanes: Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229) at 1/1000 dilution
Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: THP-1 treated first with 80nM TPA for 24 hours, then change fresh medium, transfect 10ug/ml poly(dA:dT) for 6 hours, whole cell lysate at 20 µg
Lane 3: THP-1 treated first with 80nM TPA for 24 hours, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, whole cell lysate at 20 µg
Lane 4: THP-1 treated first with 80nM TPA for 24 hours, then change fresh medium, transfect 10ug/ml poly(dA:dT) for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate at 20 µg
Lane 5: THP-1 treated first with 80nM TPA for 24 hours, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa, 124 kDa
Exposure time: 59s
![Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229), expandable thumbnail](https://content.abcam.com/products/images/sting-phospho-s366-antibody-epr29040-93-ab324229--western-blot-img446715.jpg "Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)")
Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229)
STING (phospho S366) Western blot staining using rabbit Anti-STING (phospho S366) antibody
Blocking and diluting buffer and concentration: 1% BSA/TBST.
Performed under reducing conditions.
In Western blot, ab324229 was shown to bind specifically to STING (phospho S366). Target of interest was observed at 40kDa in wild-type THP-1 cell lysates (lane 2) with no signal observed at this size in STING knockout cell line (lane 3-4) (lane 3, knockout cell line Human TMEM173 knockout THP-1 cell line ab270493 / knockout cell lysate Human TMEM173 knockout THP-1 cell lysate ab270516).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
In Western blot, Anti-STING antibody [EPR13130-55] (Anti-STING antibody [EPR13130-55] ab239074) staining at 1/1000 dilution.
All lanes: Western blot - Anti-STING (phospho S366) antibody [EPR29040-93] (ab324229) at 1/1000 dilution
Lane 1: Untreated Wild-type THP-1 (human monocytic leukemia monocyte) whole cell lysate (untreated membrane) at 20 µg
Lane 2: Wild-type THP-1 treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated STING knockout THP-1 whole cell lysate (untreated membrane) at 20 µg
Lane 4: STING knockout THP-1 treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate (untreated membrane) at 20 µg
Lane 5: Untreated Wild-type THP-1 (human monocytic leukemia monocyte) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 6: Wild-type THP-1 treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 7: Untreated STING knockout THP-1 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 8: STING knockout THP-1 treated first with 80nM TPA for 24h, then change fresh medium, transfect 10ug/ml poly(dA:dT) and treated with 10 uM MG-132 for 6 hours, 100 uM Calyculin A was then added for additional 30 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa, 124 kDa
Exposure time: 59s
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