AB96550

Anti-STIP1/STI1 antibody

Name: Anti-STIP1/STI1 antibody (ab96550) | Abcam
Brand: Abcam Limited
SKU: AB96550
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal STIP1/STI1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STIP1 aa 1-300.

View Alternative Names

Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing protein, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521, Hop, STIP1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-STIP1/STI1 antibody (AB96550)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STIP1/STI1 antibody (AB96550)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1 antibody (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled (Red) at 1/2000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody (AB96550)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody (AB96550)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1 antibody at 1/100 dilution.
Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Unknown

Western blot - Anti-STIP1/STI1 antibody (AB96550)

7.5% SDS PAGE

All lanes:

Western blot - Anti-STIP1/STI1 antibody (ab96550) at 1/5000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 63 kDa

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Supplier Data

Western blot - Anti-STIP1/STI1 antibody (AB96550)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-STIP1/STI1 antibody (ab96550) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

GL261 whole cell lysates at 30 µg

Lane 3:

C8D30 whole cell lysates at 30 µg

Lane 4:

NIH-3T3 whole cell lysates at 30 µg

Lane 5:

BCL-1 whole cell lysates at 30 µg

Lane 6:

Raw264.7 whole cell lysates at 30 µg

Lane 7:

C2C12 whole cell lysates at 30 µg

Predicted band size: 63 kDa

Observed band size: 63 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human STIP1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P31948

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STIP1 also known as STI1 is a stress-induced phosphoprotein that assists in the folding and stabilization of other proteins. The protein has a mass of approximately 63 kDa. It acts as a co-chaperone and interacts with key chaperones like HSP70 and HSP90 facilitating the transfer of client proteins between them. STIP1 is expressed in various tissues with significant presence in the nervous system and reproductive organs. It contributes to the protein homeostasis by modulating chaperone activity and protecting cells during stress conditions like high temperatures or oxidative stress.

Biological function summary

The stress-induced phosphoprotein STIP1 functions within cytoplasm and is pivotal for cellular integrity. It forms a complex with HSP70 and HSP90 that regulates the folding and activation of a range of client proteins including steroid hormone receptors and kinases. This modulation influences protein activity stability and degradation. STIP1 impacts signal transduction pathways cellular growth and stress responses by influencing how effectively client proteins perform their biological roles within cells.

Pathways

The protein STIP1 plays a significant part in the steroid hormone signaling pathway and the protein folding network. It helps facilitate the proper folding and function of steroid hormone receptors through its interaction with major chaperone proteins HSP70 and HSP90. Both these pathways are important for cell survival and proper cell regulation. By helping with the maturation and activation of client proteins STIP1 ensures that cellular responses to various internal and external signals proceed efficiently.

The involvement of STIP1 emerges most clearly in neurodegenerative conditions like Alzheimer's disease and cancer. In Alzheimer's disease STIP1 interacts with prion proteins which influence amyloid-beta aggregation and may impact disease progression. In cancer the protein assists in stabilizing client proteins essential for tumor progression such as steroid hormone receptors and protein kinases. Disruption in STIP1 function can lead to impaired cellular stress responses and may contribute to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a co-chaperone for HSP90AA1 (PubMed : 27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

See full target information STIP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 34:101539 PubMed32353747

2020

Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Mota-Martorell,Mariona Jove,Irene Pradas,Isabel Sanchez,José Gómez,Alba Naudi,Gustavo Barja,Reinald Pamplona

Pathology, research and practice 215:152564 PubMed31547977

2019

Stress-induced phosphoprotein 1 promotes pancreatic cancer progression through activation of the FAK/AKT/MMP signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanming Jing,Wenqing Liang,Jian Liu,Lin Zhang,Jianguo Wei,Yafang Zhu,Jianhui Yang,Kewei Ji,Yu Zhang,Zongliang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STIP1/STI1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-STIP1/STI1 antibody (AB96550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody (AB96550)

Western blot - Anti-STIP1/STI1 antibody (AB96550)

Western blot - Anti-STIP1/STI1 antibody (AB96550)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise