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Rabbit Recombinant Monoclonal STIP1/STI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 8 publications.


Images

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724), expandable thumbnail
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724), expandable thumbnail
  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/250- 1/500

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

For unpurified use at1/20

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal STIP1/STI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6605
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STIP1 also known as STI1 is a stress-induced phosphoprotein that assists in the folding and stabilization of other proteins. The protein has a mass of approximately 63 kDa. It acts as a co-chaperone and interacts with key chaperones like HSP70 and HSP90 facilitating the transfer of client proteins between them. STIP1 is expressed in various tissues with significant presence in the nervous system and reproductive organs. It contributes to the protein homeostasis by modulating chaperone activity and protecting cells during stress conditions like high temperatures or oxidative stress.

Biological function summary

The stress-induced phosphoprotein STIP1 functions within cytoplasm and is pivotal for cellular integrity. It forms a complex with HSP70 and HSP90 that regulates the folding and activation of a range of client proteins including steroid hormone receptors and kinases. This modulation influences protein activity stability and degradation. STIP1 impacts signal transduction pathways cellular growth and stress responses by influencing how effectively client proteins perform their biological roles within cells.

Pathways

The protein STIP1 plays a significant part in the steroid hormone signaling pathway and the protein folding network. It helps facilitate the proper folding and function of steroid hormone receptors through its interaction with major chaperone proteins HSP70 and HSP90. Both these pathways are important for cell survival and proper cell regulation. By helping with the maturation and activation of client proteins STIP1 ensures that cellular responses to various internal and external signals proceed efficiently.

Associated diseases and disorders

The involvement of STIP1 emerges most clearly in neurodegenerative conditions like Alzheimer's disease and cancer. In Alzheimer's disease STIP1 interacts with prion proteins which influence amyloid-beta aggregation and may impact disease progression. In cancer the protein assists in stabilizing client proteins essential for tumor progression such as steroid hormone receptors and protein kinases. Disruption in STIP1 function can lead to impaired cellular stress responses and may contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126724 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: STIP1 knockout HAP1 cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 63 kDa

    Observed band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution

    Lane 1: Mouse brain lysates at 15 µg

    Lane 2: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa

    Observed band size: 63 kDa

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution

    All lanes: Human brain lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 63 kDa

    Observed band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: BxPC3 cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Lane 5: SK OV 3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purifiedab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution

    All lanes: Mouse brain whole tissue lysate at 150 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa

    Observed band size: 63 kDa

    Exposure time: 5min

  • Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724)

    STIP1/STI1 western blot using anti-STIP1/STI1 antibody [EPR6605] ab126724. Publication image and figure legend from Cui, J., Reed, J., et al., 2019, Front Cell Neurosci, PubMed 31680862.


    ab126724 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126724 please see the product overview.

    Western blot analysis of proteins identified in proteomic analysis. (A) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from WT, WT/OVX, APP, and APP/OVX mice, with β-actin as a loading control. (B) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from APP/OVX, APP/OVX early treatment group and APP/OVX late treatment group, with β-actin as a loading control. (C) The levels of hemoglobin α and MAP1A were detected in the brain lysates from WT, WT/OVX, WT/OVX early treatment group and WT/OVX late treatment group, with β-actin as a loading control. ∗Indicates P < 0.05 compared to APP/OVX or between the designated two groups. ∗∗Means P < 0.01 compared to APP/OVX or designated group as indicated.

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