Rabbit Recombinant Monoclonal STIP1/STI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 8 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | WB | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/250- 1/500 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes For unpurified use at1/20 |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing protein, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521, Hop, STIP1
Rabbit Recombinant Monoclonal STIP1/STI1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 8 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
STIP1 also known as STI1 is a stress-induced phosphoprotein that assists in the folding and stabilization of other proteins. The protein has a mass of approximately 63 kDa. It acts as a co-chaperone and interacts with key chaperones like HSP70 and HSP90 facilitating the transfer of client proteins between them. STIP1 is expressed in various tissues with significant presence in the nervous system and reproductive organs. It contributes to the protein homeostasis by modulating chaperone activity and protecting cells during stress conditions like high temperatures or oxidative stress.
The stress-induced phosphoprotein STIP1 functions within cytoplasm and is pivotal for cellular integrity. It forms a complex with HSP70 and HSP90 that regulates the folding and activation of a range of client proteins including steroid hormone receptors and kinases. This modulation influences protein activity stability and degradation. STIP1 impacts signal transduction pathways cellular growth and stress responses by influencing how effectively client proteins perform their biological roles within cells.
The protein STIP1 plays a significant part in the steroid hormone signaling pathway and the protein folding network. It helps facilitate the proper folding and function of steroid hormone receptors through its interaction with major chaperone proteins HSP70 and HSP90. Both these pathways are important for cell survival and proper cell regulation. By helping with the maturation and activation of client proteins STIP1 ensures that cellular responses to various internal and external signals proceed efficiently.
The involvement of STIP1 emerges most clearly in neurodegenerative conditions like Alzheimer's disease and cancer. In Alzheimer's disease STIP1 interacts with prion proteins which influence amyloid-beta aggregation and may impact disease progression. In cancer the protein assists in stabilizing client proteins essential for tumor progression such as steroid hormone receptors and protein kinases. Disruption in STIP1 function can lead to impaired cellular stress responses and may contribute to disease pathogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126724 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: STIP1 knockout HAP1 cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: HEK-293 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution
Lane 1: Mouse brain lysates at 15 µg
Lane 2: Rat brain lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution
All lanes: Human brain lysates at 15 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1:1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: BxPC3 cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Lane 5: SK OV 3 cell lysate at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa
Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purifiedab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
All lanes: Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution
All lanes: Mouse brain whole tissue lysate at 150 µg
All lanes: HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 5min
Image collected and cropped by CiteAb under a CC-BY license from the publication
STIP1/STI1 western blot using anti-STIP1/STI1 antibody [EPR6605] ab126724. Publication image and figure legend from Cui, J., Reed, J., et al., 2019, Front Cell Neurosci, PubMed 31680862.
ab126724 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126724 please see the product overview.
Western blot analysis of proteins identified in proteomic analysis. (A) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from WT, WT/OVX, APP, and APP/OVX mice, with β-actin as a loading control. (B) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from APP/OVX, APP/OVX early treatment group and APP/OVX late treatment group, with β-actin as a loading control. (C) The levels of hemoglobin α and MAP1A were detected in the brain lysates from WT, WT/OVX, WT/OVX early treatment group and WT/OVX late treatment group, with β-actin as a loading control. ∗Indicates P < 0.05 compared to APP/OVX or between the designated two groups. ∗∗Means P < 0.01 compared to APP/OVX or designated group as indicated.
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