Anti-STIP1/STI1 antibody [EPR6605]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

Unpurified ab126724, at a dilution of 1/250, staining STIP1/STI1 in paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling STIP1/STI1 with Purified ab126724 at 1 : 1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling STIP1/STI1 with Purified ab126724 at 1 : 1000 dilution (0.18 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling STIP1/STI1 (red) with purifiedab126724 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

• WB

Lab

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

Lanes 1 - 4 : Merged signal (red and green). Green - ab126724 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab126724 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab126724 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

STIP1 knockout HAP1 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 63 kDa

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• WB

AbReview42228****

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/1000 dilution

All lanes:

Mouse brain whole tissue lysate at 150 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

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Exposure time: 5min

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution

All lanes:

Human brain lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

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• WB

Lab

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/20000 dilution

Lane 1:

Mouse brain lysates at 15 µg

Lane 2:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

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• WB

Unknown

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

All lanes:

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (ab126724) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

SK OV 3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

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• WB

CiteAb

Western blot - Anti-STIP1/STI1 antibody [EPR6605] (AB126724)

STIP1/STI1 western blot using anti-STIP1/STI1 antibody [EPR6605] ab126724. Publication image and figure legend from Cui, J., Reed, J., et al., 2019, Front Cell Neurosci, PubMed 31680862.

ab126724 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126724 please see the product overview.

Western blot analysis of proteins identified in proteomic analysis. (A) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from WT, WT/OVX, APP, and APP/OVX mice, with β-actin as a loading control. (B) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from APP/OVX, APP/OVX early treatment group and APP/OVX late treatment group, with β-actin as a loading control. (C) The levels of hemoglobin α and MAP1A were detected in the brain lysates from WT, WT/OVX, WT/OVX early treatment group and WT/OVX late treatment group, with β-actin as a loading control. *Indicates p < 0.05 compared to APP/OVX or between the designated two groups. **Means p < 0.01 compared to APP/OVX or designated group as indicated.

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