AB71960

Anti-STK3/MST-2 antibody [3067C3a]

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(2 Publications)

Mouse Monoclonal STK3/MST-2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STK3.

View Alternative Names

KRS1, MST2, STK3, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, MST-2

3 Images

Lab

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : STK3/MST-2 knockout HAP1 cell lysate (20 μg)
Lane 3 : NIH/3T3 cell lysate (20 μg)
Lane 4 : 293T cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71960 was shown to recognize STK3/MST-2 when STK3/MST-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab71960 and ab181602 (loading control to STK3/MST-2) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)

Predicted band size: 56 kDa

false

Unknown

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

All lanes:

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960)

All lanes:

immunising recombinant protein

Predicted band size: 56 kDa

Observed band size: 37 kDa

false

Lab

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

Lanes 1- 2 : Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

ab71960 was shown to react with STK3/MST-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265082 (knockout cell lysate ab257714) was used. Wild-type HeLa and STK3 knockout HeLa cell lysates were subjected to SDS-PAGE. ab71960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (ab71960) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

STK3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human STK3 (MST-2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-stk3-mst-2-knockout-hela-cell-line-ab265082'>ab265082</a>)

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3067C3a

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human STK3. The exact immunogen used to generate this antibody is proprietary information.

Q13188

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab71960 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STK3 also known as MST-2 is a serine/threonine protein kinase with a molecular mass of around 60 kDa. It plays an integral role in cell signaling by phosphorylating and activating downstream targets. MST-2 localizes mainly in the cytoplasm and is expressed broadly across various tissues with notable activity in liver kidney and heart tissues. This kinase contributes to the regulation of cell shape and apoptosis reinforcing its importance in cellular homeostasis.

Biological function summary

STK3/MST-2 is a core component of the Hippo signaling pathway which controls organ size by regulating cell proliferation and apoptosis. MST-2 often interacts with other proteins and participates in functional complexes notably with MOB1 and LATS1/2. These interactions are essential for transmitting growth inhibitory signals. Through its kinase activity MST-2 phosphorylates and activates these molecules initiating a cascade that ultimately influences gene expression impacting cell cycle and cell death processes.

Pathways

MST-2 plays a pivotal role not only in the Hippo pathway but also in the MAPK signaling network. In the Hippo pathway MST-2 phosphorylates components like LATS1/2 subsequently affecting the translocation and activity of YAP/TAZ transcription factors which control cell proliferation and survival. In the MAPK pathway MST-2 can interact with other kinases such as ASK1 to influence stress response signaling. These interactions demonstrate how MST-2 integrates signals between pathways to mediate critical decisions in cell life and death.

MST-2 has implications in cancer and cardiac diseases. Dysregulation of the Hippo pathway involving MST-2 is frequently observed in various cancers where impaired MST-2 function contributes to unchecked cell growth and tumor progression. Moreover MST-2 connects with other proteins like YAP often overactive in cancers emphasizing its role in controlling cell division. In cardiac context alterations in MST-2 activity relate to cardiac hypertrophy as proper functioning of the kinase is necessary to maintain cardiac tissue architecture and response to stress.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed : 11278283, PubMed : 8566796, PubMed : 8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 15688006, PubMed : 16930133, PubMed : 23972470, PubMed : 28087714, PubMed : 29063833, PubMed : 30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed : 15688006, PubMed : 16930133, PubMed : 23972470, PubMed : 28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed : 21076410, PubMed : 21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed : 21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed : 20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed : 19525978). Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed : 18328708, PubMed : 18362890).

See full target information STK3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 30:1208-1222.e9 PubMed31995759

2020

PRMT1 Is Recruited via DNA-PK to Chromatin Where It Sustains the Senescence-Associated Secretory Phenotype in Response to Cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Musiani,Roberto Giambruno,Enrico Massignani,Marica Rosaria Ippolito,Marianna Maniaci,Sriganesh Jammula,Daria Manganaro,Alessandro Cuomo,Luciano Nicosia,Diego Pasini,Tiziana Bonaldi

The EMBO journal 38:e101168 PubMed31414556

2019

RASSF1A is required for the maintenance of nuclear actin levels.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Chatzifrangkeskou,Dafni-Eleftheria Pefani,Michael Eyres,Iolanda Vendrell,Roman Fischer,Daniela Pankova,Eric O'Neill

View all publications

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Product promise

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Anti-STK3/MST-2 antibody [3067C3a]

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

Western blot - Anti-STK3/MST-2 antibody [3067C3a] (AB71960)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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