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Rabbit Recombinant Monoclonal STK3/MST-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 39 publications.

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Images

Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641), expandable thumbnail
  • Immunoprecipitation - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641), expandable thumbnail
  • Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Tested
Expected
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
5 µg/mL
Notes

-

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/70
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Antigen retrieval step strongly recommended for enhanced signal.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed:11278283, PubMed:8566796, PubMed:8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714, PubMed:29063833, PubMed:30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed:21076410, PubMed:21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed:21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed:20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed:19525978). Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed:18328708, PubMed:18362890).

Alternative names

KRS1, MST2, STK3, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, MST-2

Recommended products

Rabbit Recombinant Monoclonal STK3/MST-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 39 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1466Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STK3 also known as MST-2 is a serine/threonine protein kinase with a molecular mass of around 60 kDa. It plays an integral role in cell signaling by phosphorylating and activating downstream targets. MST-2 localizes mainly in the cytoplasm and is expressed broadly across various tissues with notable activity in liver kidney and heart tissues. This kinase contributes to the regulation of cell shape and apoptosis reinforcing its importance in cellular homeostasis.

Biological function summary

STK3/MST-2 is a core component of the Hippo signaling pathway which controls organ size by regulating cell proliferation and apoptosis. MST-2 often interacts with other proteins and participates in functional complexes notably with MOB1 and LATS1/2. These interactions are essential for transmitting growth inhibitory signals. Through its kinase activity MST-2 phosphorylates and activates these molecules initiating a cascade that ultimately influences gene expression impacting cell cycle and cell death processes.

Pathways

MST-2 plays a pivotal role not only in the Hippo pathway but also in the MAPK signaling network. In the Hippo pathway MST-2 phosphorylates components like LATS1/2 subsequently affecting the translocation and activity of YAP/TAZ transcription factors which control cell proliferation and survival. In the MAPK pathway MST-2 can interact with other kinases such as ASK1 to influence stress response signaling. These interactions demonstrate how MST-2 integrates signals between pathways to mediate critical decisions in cell life and death.

Associated diseases and disorders

MST-2 has implications in cancer and cardiac diseases. Dysregulation of the Hippo pathway involving MST-2 is frequently observed in various cancers where impaired MST-2 function contributes to unchecked cell growth and tumor progression. Moreover MST-2 connects with other proteins like YAP often overactive in cancers emphasizing its role in controlling cell division. In cardiac context alterations in MST-2 activity relate to cardiac hypertrophy as proper functioning of the kinase is necessary to maintain cardiac tissue architecture and response to stress.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: STK3/MST-2 knockout HAP1 cell lysate (20 μg)
    Lane 3: NIH/3T3 cell lysate (20 μg)
    Lane 4: 293T cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab52641 observed at 56 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab52641 was shown to specifically react with STK3/MST-2 when STK3/MST-2 knockout samples were used. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab52641 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    ab52641 staining STK3/MST-2 in wild-type HAP1 cells (top panel) and STK3/MST-2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52641 at 5μg/ml concentration and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab52641 at a working dilution of 1/50. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunoprecipitation - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Immunoprecipitation - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    ab52641 (purified) at 1/50 immunoprecipitating STK3/MST-2 in 10 μg C6 cell lysate (Lanes 1 and 2, observed at 56 kDa). Lane 3 - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). For western blotting, HRP Veriblot for IP (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Predicted band size: 56 kDa

  • Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641) at 1/50000 dilution

    Lane 1: C6 whole cell lysate at 10 µg

    Lane 2: NIH/3T3 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Confocal image showing cytoplasmic staining of STK3/MST-2 in NIH/3T3 (mouse embryonic fibroblast) cells using ab52641 . The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. The cells were then incubated with ab52641 at 1/70 dilution and counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/100 dilution (red). Goat anti Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

  • Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641) at 1/50000 dilution

    Lane 1: HEK293 whole cell lysate at 10 µg

    Lane 2: HeLa whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Flow Cytometry (Intracellular) - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STK3/MST-2 antibody [EP1466Y] (ab52641)

    NIH/3T3 (Mouse embryonic fibroblast) cells were fixed with4% paraformaldehyde and permeabilised with 90% methanol. The primary antibody (ab52641) was used at a 1/70 dilution (1 μg) (red). A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. A Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) was used as an isotype control.Cells without incubation with primary antibody and secondary antibody were used as an unlabelled control (blue).

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