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Rabbit Recombinant Monoclonal STK33 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-STK33 antibody [EPR15343] (AB206296), expandable thumbnail
  • Western blot - Anti-STK33 antibody [EPR15343] (AB206296), expandable thumbnail
  • Western blot - Anti-STK33 antibody [EPR15343] (AB206296), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (AB206296), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (AB206296), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Serine/threonine protein kinase required for spermatid differentiation and male fertility (PubMed:37146716, PubMed:38781365). Promotes sperm flagella assembly during spermatogenesis by mediating phosphorylation of fibrous sheath proteins AKAP3 and AKAP4 (By similarity). Also phosphorylates VIME, thereby regulating the dynamic behavior of the intermediate filament cytoskeleton (By similarity).

Alternative names

Serine/threonine-protein kinase 33, STK33

Recommended products

Rabbit Recombinant Monoclonal STK33 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15343
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STK33 also known as serine/threonine kinase 33 is a nucleic protein kinase with a mass of approximately 59 kDa. The STK33 protein functions by phosphorylating serine and threonine amino acid residues which helps to regulate various cellular processes. It is broadly expressed in different tissues such as the brain lung and liver indicating its widespread importance in cellular functions.

Biological function summary

STK33 plays a role in cell proliferation and survival. While not part of any well-defined complex STK33 affects pathways associated with tumorigenic processes. It supports cellular growth by promoting cell cycle progression and inhibiting apoptosis which can lead to increased cell proliferation. These functions highlight its potential involvement in oncogenic events where dysregulation of cell growth is a common feature.

Pathways

STK33 is involved in the PI3K/AKT signaling pathway an important pathway for cell survival and metabolism. In this context STK33 interacts with proteins such as AKT and mTOR which are key components of the pathway. Additionally STK33 has connections with the MAPK/ERK pathway linking it with proteins like MEK and ERK well-known for their roles in cell proliferation and differentiation.

Associated diseases and disorders

STK33 frequently associates with various types of cancer including lung and pancreatic cancer. Its role in promoting cell cycle progression and inhibiting apoptosis associates it with tumor survival and growth. In cancer STK33 activity connects with oncogenic proteins like KRAS influencing cancer cell metabolism and proliferation. Understanding STK33's involvement in these pathways and diseases may offer new avenues for targeted therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)

    Blocking/dilution buffer: 5% NFDM/TBST.

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

    All lanes: Western blot - Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

    Exposure time: 3min

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)

    Blocking/dilution buffer: 5% NFDM/TBST.

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively

    All lanes: Western blot - Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    All lanes: Human testis tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58 kDa

    Observed band size: 50 kDa, 58 kDa

    Exposure time: 1min

  • Western blot - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Western blot - Anti-STK33 antibody [EPR15343] (ab206296)

    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds;
    Lane 2: 3 minutes

    All lanes: Western blot - Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1: Human fetal kidney tissue lysate at 10 µg

    Lane 2: Human fetal spleen tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human testis tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunohistochemical analysis of paraffin-embedded human fetal cardiac tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nuclear and cytoplasmic staining on human fetal cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-STK33 antibody [EPR15343] (ab206296), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STK33 antibody [EPR15343] (ab206296)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STK33 with ab206296 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and nuclear staining on Jurkat cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab206296 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2. - anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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