Anti-STK33 antibody [EPR15343] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STK33 with ab206296 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic and nuclear staining on Jurkat cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 - ab206296 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2. - anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and cytoplasmic staining on human testis tissue is observed.

Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human fetal cardiac tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear and cytoplasmic staining on human fetal cardiac muscle tissue is observed.

Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 seconds; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-STK33 antibody [EPR15343] (<a href='/en-us/products/primary-antibodies/stk33-antibody-epr15343-ab206296'>ab206296</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively.

All lanes:

Western blot - Anti-STK33 antibody [EPR15343] (<a href='/en-us/products/primary-antibodies/stk33-antibody-epr15343-ab206296'>ab206296</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 50 kDa,58 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (AB251446)

This data was developed using ab206296, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively.

All lanes:

Western blot - Anti-STK33 antibody [EPR15343] (<a href='/en-us/products/primary-antibodies/stk33-antibody-epr15343-ab206296'>ab206296</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

Exposure time: 3min