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AB224257

Anti-STMN3 antibody

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(1 Publication)

Rabbit Polyclonal STMN3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STMN3 aa 50-200.

View Alternative Names

SCLIP, STMN3, Stathmin-3, SCG10-like protein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)

Immunohistochemical analysis of human testis tissue labeling STMN3 with ab224257 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)

Immunohistochemical analysis of human cerebral cortex tissue labeling STMN3 with ab224257 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)

Immunohistochemical analysis of human cerebral cortex tissue labeling STMN3 in the cytoplasm of neurons with ab224257 at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STMN3 antibody (AB224257)

Immunohistochemical analysis of human skeletal muscle tissue labeling STMN3 with ab224257 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-STMN3 antibody (AB224257)
  • WB

Supplier Data

Western blot - Anti-STMN3 antibody (AB224257)

All lanes:

Western blot - Anti-STMN3 antibody (ab224257) at 0.4 µg/mL

Lane 1:

Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

STMN3 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human STMN3 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NZ72

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Complementary Products

Proteins & Peptides

AB97900

Recombinant Human STMN3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human STMN3 protein (His tag N-Terminus) (AB97900)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stathmin 3 also known as STMN3 is a microtubule-destabilizing protein with a molecular mass of approximately 18 kDa. The protein plays a mechanical role in the regulation of the microtubule network by promoting microtubule depolymerization which is essential for cellular processes involving microtubule dynamics. STMN3 is prominently expressed in neural tissues and contributes to the regulation of the cytoskeleton during neuron development and differentiation.
Biological function summary

The regulation of microtubule dynamics through STMN3 affects neuronal growth and synaptic plasticity which are key for brain function. STMN3 does not operate as part of a large protein complex like some other microtubule-associated proteins but its activity is modulated by phosphorylation states that influence its ability to bind to tubulin and destabilize microtubules.

Pathways

Several processes involving STMN3 include the cellular cytoskeleton organization and neural cell signaling pathways. The protein's function is closely tied to the PI3K/AKT signaling pathway which modulates its activity through phosphorylation. STMN3 interacts with tubulin subunits impacting microtubule dynamics that in turn influence cell signaling and transport processes within neurons.

Dysregulation of STMN3 has connections to neurological disorders such as Alzheimer's disease and schizophrenia. Alterations in STMN3 expression or function can affect microtubule stability and neuronal function which contribute to the pathophysiology of these conditions. Related proteins like tau in Alzheimer's disease also interact with microtubules and abnormal modifications can lead to compromised neuronal integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits microtubule-destabilizing activity, which is antagonized by STAT3.
See full target information STMN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6446 PubMed37833281

2023

Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitali Chakraborty,Itzel Nissen,Craig A Vincent,Anna-Carin Hägglund,Andreas Hörnblad,Silvia Remeseiro
View all publications
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Product promise

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