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AB169524

Anti-Stomatin antibody [EPR10420]

5

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal Stomatin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

BND7, EPB72, STOM, Stomatin, Erythrocyte band 7 integral membrane protein, Erythrocyte membrane protein band 7.2, Protein 7.2b

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (AB169524)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (AB169524)

Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10420] (AB169524)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10420] (AB169524)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-Stomatin antibody [EPR10420] (AB169524)
  • WB

Unknown

Western blot - Anti-Stomatin antibody [EPR10420] (AB169524)

All lanes:

Western blot - Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Western blot - Anti-Stomatin antibody [EPR10420] (AB169524)
  • WB

Unknown

Western blot - Anti-Stomatin antibody [EPR10420] (AB169524)

All lanes:

Western blot - Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from HepG2 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 32 kDa

false

  • Carrier free

    Anti-Stomatin antibody [EPR10420] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10420

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
See full target information STOM

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 601:1383-1405 PubMed36864773

2023

Short-term high-fat diet feeding of mice suppresses catecholamine-stimulated Ca signalling in hepatocytes and intact liver.

Applications

Unspecified application

Species

Unspecified reactive species

Robert P Brumer,Juliana C Corrêa-Velloso,Samantha J Thomas,Oleta A Sandiford,Andrew P Thomas,Paula J Bartlett

Bioactive materials 11:1-14 PubMed34938908

2021

Targeted core-shell nanoparticles for precise CTCF gene insert in treatment of metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jialun Duan,Chunjie Bao,Ying Xie,Haitao Guo,Yixuan Liu,Jianwei Li,Rui Liu,Peishan Li,Jing Bai,Yan Yan,Limin Mu,Xueqi Li,Guiling Wang,Wanliang Lu

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 31:930-944 PubMed31949395

2020

Stomatin plays a suppressor role in non-small cell lung cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaying An,Xiao Ma,Mingyi Liu,Xiaotong Wang,Xundong Wei,Wei Yuan,Jie Ma
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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