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Rabbit Recombinant Monoclonal Stomatin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-Stomatin antibody [EPR10420] (AB169524), expandable thumbnail
  • Western blot - Anti-Stomatin antibody [EPR10420] (AB169524), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (AB169524), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10420] (AB169524), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Stomatin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10420
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Stomatin antibody [EPR10420] (ab169524), expandable thumbnail

    Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)

    All lanes: Western blot - Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-Stomatin antibody [EPR10420] (ab169524), expandable thumbnail

    Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)

    All lanes: Western blot - Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

    All lanes: immunoprecipitation pellet from HepG2 cell lysate

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (ab169524), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (ab169524)

    Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).

  • Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10420] (ab169524), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10420] (ab169524)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com