AB166623

Anti-Stomatin antibody [EPR10421]

RabMAb
Recombinant
What is this?

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(4 Publications )

Rabbit Recombinant Monoclonal Stomatin antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

BND7, EPB72, STOM, Stomatin, Erythrocyte band 7 integral membrane protein, Erythrocyte membrane protein band 7.2, Protein 7.2b

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (AB166623)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (AB166623)

Immunofluorescence analysis of HeLa cells labeling Stomatin with ab166623 at 1/100 dilution.

Unknown

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (AB166623)

Immunoprecipitation of Stomatin in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of Stomatin.

All lanes:

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from Human fetal heart lysate at 10 µg

Predicted band size: 32 kDa

false

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10421] (AB166623)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10421] (AB166623)

Intracellular flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-Stomatin antibody [EPR10421] (AB166623)

All lanes:

Western blot - Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Neuro-2a lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Predicted band size: 32 kDa

false

Carrier free

Anti-Stomatin antibody [EPR10421] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10421

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.

See full target information STOM

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

mSphere 8:e0052322 PubMed36719247

2023

The Lipid Raft-Associated Protein Stomatin Is Required for Accumulation of Dectin-1 in the Phagosomal Membrane and for Full Activity of Macrophages against Aspergillus fumigatus.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Goldmann,Franziska Schmidt,Zoltán Cseresnyés,Thomas Orasch,Susanne Jahreis,Susann Hartung,Marc Thilo Figge,Marie von Lilienfeld-Toal,Thorsten Heinekamp,Axel A Brakhage

Neurobiology of stress 22:100513 PubMed36636173

2023

Proteomic analysis identifies Stomatin as a biological marker for psychological stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Cao,Si-Qi Ying,Xin-Yu Qiu,Jia Guo,Chen Chen,Shi-Jie Li,Geng Dou,Chen-Xi Zheng,Da Chen,Ji-Yu Qiu,Yan Jin,Bing-Dong Sui,Fang Jin

Experimental and therapeutic medicine 23:69 PubMed34934440

2021

Stomatin-knockdown effectively attenuates sepsis-induced oxidative stress and inflammation of alveolar epithelial cells by regulating CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Wu,Li Wang

eLife 4: PubMed25803486

2015

Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Laurette,Thomas Strub,Dana Koludrovic,Céline Keime,Stéphanie Le Gras,Hannah Seberg,Eric Van Otterloo,Hana Imrichova,Robert Siddaway,Stein Aerts,Robert A Cornell,Gabrielle Mengus,Irwin Davidson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Stomatin antibody [EPR10421]

Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (AB166623)

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (AB166623)

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10421] (AB166623)

Western blot - Anti-Stomatin antibody [EPR10421] (AB166623)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise