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AB249361

Anti-Stomatin antibody [EPR10421] - BSA and Azide free

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Rabbit Recombinant Monoclonal Stomatin antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

View Alternative Names

BND7, EPB72, STOM, Stomatin, Erythrocyte band 7 integral membrane protein, Erythrocyte membrane protein band 7.2, Protein 7.2b

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)

This data was developed using ab166623, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling Stomatin with ab166623 at 1/100 dilution.

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)
  • IP

Unknown

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)

This data was developed using ab166623, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Stomatin in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by western blot analysis of Stomatin.

All lanes:

Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (<a href='/en-us/products/primary-antibodies/stomatin-antibody-epr10421-ab166623'>ab166623</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from Human fetal heart lysate at 10 µg

Predicted band size: 32 kDa

false

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)

This data was developed using ab166623, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

Western blot - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)
  • WB

Unknown

Western blot - Anti-Stomatin antibody [EPR10421] - BSA and Azide free (AB249361)

This data was developed using ab166623, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Stomatin antibody [EPR10421] (<a href='/en-us/products/primary-antibodies/stomatin-antibody-epr10421-ab166623'>ab166623</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Neuro-2a lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10421

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249361 is the carrier-free version of ab166623.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
See full target information STOM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com