Anti-Stra8 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (AB49405)

IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Unknown

Western blot - Anti-Stra8 antibody (AB49405)

ab49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.

All lanes:

Western blot - Anti-Stra8 antibody (ab49405) at 1 µg/mL

Lane 1:

CHO overexpressing Mouse Stra8 lysate at 1 µL

Lane 2:

CHO Negative Control Lysate at 1 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 60 kDa

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Exposure time: 1min