Rabbit Polyclonal STRA8 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 40 publications.
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- BMC biology 21:2812023FGF-independent MEK1/2 signalling in the developing foetal testis is essential for male germline differentiation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRheannon O Blücher,Rachel S Lim,Ellen G Jarred,Matthew E Ritchie,Patrick S WesternPubMed 38053127
- Cell death & disease 14:1342023Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFa-Li Zhang,Yan-Qin Feng,Jing-Ya Wang,Ke-Xin Zhu,Lu Wang,Jia-Mao Yan,Xiu-Xiu Li,Jun-Jie Wang,Wei Ge,Massimo De Felici,Wei ShenPubMed 36797258
- Nature communications 13:79532022Diverse monogenic subforms of human spermatogenic failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiina Nagirnaja,Alexandra M Lopes,Wu-Lin Charng,Brian Miller,Rytis Stakaitis,Ieva Golubickaite,Alexandra Stendahl,Tianpengcheng Luan,Corinna Friedrich,Eisa Mahyari,Eloise Fadial,Laura Kasak,Katinka Vigh-Conrad,Manon S Oud,Miguel J Xavier,Samuel R Cheers,Emma R James,Jingtao Guo,Timothy G Jenkins,Antoni Riera-Escamilla,Alberto Barros,Filipa Carvalho,Susana Fernandes,João Gonçalves,Christina A Gurnett,Niels Jørgensen,Davor Jezek,Emily S Jungheim,Sabine Kliesch,Robert I McLachlan,Kenan R Omurtag,Adrian Pilatz,Jay I Sandlow,James Smith,Michael L Eisenberg,James M Hotaling,Keith A Jarvi,Margus Punab,Ewa Rajpert-De Meyts,Douglas T Carrell,Csilla Krausz,Maris Laan,Moira K O'Bryan,Peter N Schlegel,Frank Tüttelmann,Joris A Veltman,Kristian Almstrup,Kenneth I Aston,Donald F ConradPubMed 36572685
- Life science alliance 6:2022Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanze Song,Min Chen,Yingfan Zhang,Jiayi Li,Bowen Liu,Na Li,Min Chen,Miaomiao Qiao,Nan Wang,Yuanwei Cao,Shan Lu,Jian Chen,Wen Sun,Fei Gao,Haoyi WangPubMed 36414375
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e225222022The function of Foxo1 in spermatogonia development is independent of PI3K/PTEN signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiming Shen,Min Chen,Yang Gao,Fangfang Dong,Changhuo Cen,Haowei Wu,Nan Wang,Xiuhong Cui,Chunsheng Han,Fei GaoPubMed 36052752
- Genes 12:2021DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodolphe Dard,Manon Moreau,Estelle Parizot,Farah Ghieh,Leslie Brehier,Nadim Kassis,Valérie Serazin,Antonin Lamaziere,Chrystèle Racine,Nathalie di Clemente,François Vialard,Nathalie JanelPubMed 34828406
- Frontiers in genetics 12:7524952021A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWensheng Liu,Yinan Zhao,Xiaohua Liu,Xiaoya Zhang,Jiancheng Ding,Yang Li,Yingpu Tian,Haibin Wang,Wen Liu,Zhongxian LuPubMed 34707642
- Frontiers in cell and developmental biology 9:6732582021PRMT5 Is Involved in Spermatogonial Stem Cells Maintenance by Regulating Expression via Modulation of Lysine Histone Modifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangfang Dong,Min Chen,Min Chen,Lin Jiang,Zhiming Shen,Longfei Ma,Chunsheng Han,Xudong Guo,Fei GaoPubMed 34113620
- Journal of cellular and molecular medicine 25:383-3962020Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c-KIT expression in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Giovanna Desimio,Eleonora Cesari,Maria Sorrenti,Massimo De Felici,Donatella FariniPubMed 33236849
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12634-126452020Single-cell RNA sequencing reveals the landscape of early female germ cell development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng-Hui Zhao,Jun-Yu Ma,Tie-Gang Meng,Zhen-Bo Wang,Wei Yue,Qian Zhou,Sen Li,Xie Feng,Yi Hou,Heide Schatten,Xiang-Hong Ou,Qing-Yuan SunPubMed 32716582
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1 µg/mL | Notes Recommended use at a low concentration otherwise high background staining may be observed |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Meiosis-inducer required for the transition into meiosis for both female and male germ cells (PubMed:16461896, PubMed:17115059, PubMed:18799751, PubMed:18799790, PubMed:19805549, PubMed:32032549, PubMed:32054698). In female germ cells, acts downstream of ZGLP1 as a key effector of the meiotic program: required for premeiotic DNA replication and subsequent events in meiotic prophase (PubMed:16461896, PubMed:17115059, PubMed:18799751, PubMed:18799790, PubMed:32054698). During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation (PubMed:25902548). In complex with MEIOSIN, directly activates the transcription of a subset of critical meiotic genes playing a central role in cell-cycle switching from mitosis to meiosis (PubMed:19805549, PubMed:32032549).
Alternative names
Stimulated by retinoic acid gene 8 protein, Stra8
Recommended products
Rabbit Polyclonal STRA8 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 40 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Stra8 antibody (ab49405)
ab49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.
All lanes: Western blot - Anti-Stra8 antibody (ab49405) at 1 µg/mL
Lane 1: CHO overexpressing Mouse Stra8 lysate at 1 µL
Lane 2: CHO Negative Control Lysate at 1 µL
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 60 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49405)
IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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