Rabbit Polyclonal STRA8 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 83 publications.
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- Molecules (Basel, Switzerland) 28:2023Protopine Alleviates Dextran Sodium Sulfate-Induced Ulcerative Colitis by Improving Intestinal Barrier Function and Regulating Intestinal Microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeishan Yue,Jialu Huang,Xiaolan Ma,Peng Huang,Yisong Liu,Jianguo ZengPubMed 37446938
- Cells 12:2023Ectopically Expressed Meiosis-Specific Cancer Testis Antigen HORMAD1 Promotes Genomic Instability in Squamous Cell Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Gantchev,Julia Messina-Pacheco,Amelia Martínez Villarreal,Brandon Ramchatesingh,Philippe Lefrançois,Pingxing Xie,Laetitia Amar,Hong Hao Xu,Keerthenan Raveendra,Daniel Sikorski,Daniel Josue Guerra Ordaz,Raman Preet Kaur Gill,Marine Lambert,Ivan V LitvinovPubMed 37371097
- The Journal of biological chemistry 299:1047832023Fat mass and obesity-associated factor (FTO)-mediated N6-methyladenosine regulates spermatogenesis in an age-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Wu,Jincheng Li,Chenmeijie Li,Shuai Lu,Xiaoyu Wei,Yang Li,Wenjuan Xia,Chunfeng Qian,Zihang Wang,Mingxi Liu,Yayun Gu,Boxian Huang,Yueqiu Tan,Zhibin HuPubMed 37146971
- Development (Cambridge, England) 150:2023Sertoli cells are the source of stem cell factor for spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Jacky Peng,Xinyu Thomas Tang,Hui Sophie Shu,Wenjie Dong,Hongfang Shao,Bo O ZhouPubMed 36861441
- Cell death & disease 14:1342023Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFa-Li Zhang,Yan-Qin Feng,Jing-Ya Wang,Ke-Xin Zhu,Lu Wang,Jia-Mao Yan,Xiu-Xiu Li,Jun-Jie Wang,Wei Ge,Massimo De Felici,Wei ShenPubMed 36797258
- Development (Cambridge, England) 149:2022Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOleksandr Kirsanov,Taylor Johnson,Taylor Malachowski,Bryan A Niedenberger,Emma A Gilbert,Debajit Bhowmick,P Hande Ozdinler,Douglas A Gray,Kelsey Fisher-Wellman,Brian P Hermann,Christopher B GeyerPubMed 36250451
- PLoS genetics 18:e10104162022The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatoya J Peart,Taylor A Johnson,Sungkyoung Lee,Matthew J Sears,Fang Yang,Mathieu Quesnel-Vallières,Huijuan Feng,Yocelyn Recinos,Yoseph Barash,Chaolin Zhang,Brian P Hermann,P Jeremy Wang,Christopher B Geyer,Russ P CarstensPubMed 36129965
- Journal of veterinary science 23:e352022Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqi Cao,ZiFu Dai,Huizhen Lao,Huimin ZhaoPubMed 35363442
- Cell reports 37:1098852021Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Heinrich,Bidur Bhandary,Sarah J Potter,Nancy Ratner,Tony DeFalcoPubMed 34706238
- Frontiers in endocrinology 12:5841252021Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengxiang Tian,Zhiming Zhao,Yanli Fan,Na Cui,Baojun Shi,Guimin HaoPubMed 34707565
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Hamster | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1 µg/mL | Notes Recommended use at a low concentration otherwise high background staining may be observed |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Meiosis-inducer required for the transition into meiosis for both female and male germ cells (PubMed:16461896, PubMed:17115059, PubMed:18799751, PubMed:18799790, PubMed:19805549, PubMed:32032549, PubMed:32054698). In female germ cells, acts downstream of ZGLP1 as a key effector of the meiotic program: required for premeiotic DNA replication and subsequent events in meiotic prophase (PubMed:16461896, PubMed:17115059, PubMed:18799751, PubMed:18799790, PubMed:32054698). During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation (PubMed:25902548). In complex with MEIOSIN, directly activates the transcription of a subset of critical meiotic genes playing a central role in cell-cycle switching from mitosis to meiosis (PubMed:19805549, PubMed:32032549).
Alternative names
Stimulated by retinoic acid gene 8 protein, Stra8
Recommended products
Rabbit Polyclonal STRA8 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 83 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49602)
IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Stra8 antibody (ab49602)
ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.
All lanes: Western blot - Anti-Stra8 antibody (ab49602) at 1 µg/mL
Lane 1: CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate at 1 µL
Lane 2: CHO Negative Control Whole Cell Lysate at 1 µL
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 60 kDa
Exposure time: 1min
This image is courtesy of an anonymous customer review.Western blot - Anti-Stra8 antibody (ab49602)
All lanes: Western blot - Anti-Stra8 antibody (ab49602) at 1/500 dilution
Lane 1: WT D3 mouse ES cells cultured with LIF. at 200000 Cells
Lane 2: WT D3 mouse ES cells cultured without LIF for 24 hours. at 200000 Cells
Lane 3: WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours. at 200000 Cells
SecondaryAll lanes: Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 48 kDa
Exposure time: 1min
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