Anti-Stra8 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (AB49602)

IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49602, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

AbReview32350****

Western blot - Anti-Stra8 antibody (AB49602)

All lanes:

Western blot - Anti-Stra8 antibody (ab49602) at 1/500 dilution

Lane 1:

WT D3 mouse ES cells cultured with LIF. at 200000 Cells

Lane 2:

WT D3 mouse ES cells cultured without LIF for 24 hours. at 200000 Cells

Lane 3:

WT D3 mouse ES cells cultured without LIF and with 1uM Retinoic acid for 24 hours. at 200000 Cells

Secondary

All lanes:

Non-Abcam antibody was used: goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 48 kDa

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Exposure time: 1min

This image is courtesy of an anonymous abreview.

• WB

Project

Western blot - Anti-Stra8 antibody (AB49602)

ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.

All lanes:

Western blot - Anti-Stra8 antibody (ab49602) at 1 µg/mL

Lane 1:

CHO Overexpressing Stra8 (Mouse) Whole Cell Lysate at 1 µL

Lane 2:

CHO Negative Control Whole Cell Lysate at 1 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 37 kDa

Observed band size: 60 kDa

true

Exposure time: 1min