Anti-STRAD antibody [EPR15603] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (AB232340)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling STRADantibody (red) with purified ab192879 at a dilution of 1/200. Goat anti rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STRAD antibody [EPR15603] - BSA and Azide free (AB232340)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling STRAD with ab192879 at a 1/400 dilution, followed by Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain : Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (AB232340)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STRAD antibody [EPR15603] - BSA and Azide free (AB232340)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192879).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-STRAD antibody [EPR15603] - BSA and Azide free (AB232340)

This data was developed using ab192879, the same antibody clone in a different buffer formulation.

Western blot : Anti-STRAD antibody [EPR15603] ab192879 staining at 1/5000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 48 kDa in Wild-type A549 cell lysates with no signal observed at this size in STRADA knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 5:

Western blot - Anti-STRAD antibody [EPR15603] (<a href='/en-us/products/primary-antibodies/strad-antibody-epr15603-ab192879'>ab192879</a>) at 1/5000 dilution

Lanes 1 - 5:

Western blot - Anti-STRAD antibody [EPR15603] - BSA and Azide free (ab232340) at 1/5000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

STRADA knockout A549 at 20 µg

Lane 3:

MCF7 at 20 µg

Lane 4:

U-87 MG at 20 µg

Lane 5:

HT-29 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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