Rabbit Recombinant Monoclonal Strep-tag II antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, WB and reacts with Tag samples.
Images
![Western blot - Anti-Strep epitope Tag antibody [HL1251] (AB308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--western-blot-img441345.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Strep epitope Tag antibody [HL1251] (AB308418)")
![Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (AB308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunoprecipitation-img408266.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (AB308418)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep epitope Tag antibody [HL1251] (AB308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep epitope Tag antibody [HL1251] (AB308418)")
![Western blot - Anti-Strep epitope Tag antibody [HL1251] (AB308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--western-blot-img408265.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Strep epitope Tag antibody [HL1251] (AB308418)")
![Immunocytochemistry/ Immunofluorescence - Anti-Strep epitope Tag antibody [HL1251] (AB308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunocytochemistry-immunofluorescence-img408263.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Strep epitope Tag antibody [HL1251] (AB308418)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | IHC-P | ICC/IF | WB | |
|---|---|---|---|---|
| Tag | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
Target data
Alternative names
Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II
Recommended products
Rabbit Recombinant Monoclonal Strep-tag II antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, WB and reacts with Tag samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1251
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--western-blot-img441345.jpg "Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418)")
Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418)
Samples were seperated by 12% SDS-PAGE.
All lanes: Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418) at 1/1000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (ab308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunoprecipitation-img408266.jpg "Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (ab308418)")
Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (ab308418)
Immunoprecipitation of Strep epitope Tag protein from Strep-tag tagged protein transfected 293T whole cell extracts using 5 μg of Strep epitope Tag antibody [HL1251] (ab308418). Western blot analysis was performed using Strep epitope Tag antibody [HL1251] (ab308418). EasyBlot HRP-conjugated anti FALSE.
All lanes: Immunoprecipitation - Anti-Strep epitope Tag antibody [HL1251] (ab308418) at 5 µg
Lane 1: 293T whole cell extracts
Lane 2: 293T whole cell extracts with IgG
Lane 3: Strep-tag tagged protein transfected 293T whole cell extracts
SecondaryAll lanes: EasyBlot HRP-conjugated anti FALSE
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep epitope Tag antibody [HL1251] (ab308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep epitope Tag antibody [HL1251] (ab308418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep epitope Tag antibody [HL1251] (ab308418)
Immunohistochemical analysis of mock and Strep Tagged protein transfected HEK293T cell pellet labeling Strep epitope Tag using ab308418 at 1/1000 dilution (green). Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--western-blot-img408265.jpg "Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418)")
Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1251] (ab308418) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Strep epitope Tag antibody [HL1251] (ab308418) at 1/1000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunocytochemistry/ Immunofluorescence - Anti-Strep epitope Tag antibody [HL1251] (ab308418), expandable thumbnail](https://content.abcam.com/products/images/strep-epitope-tag-antibody-hl1251-ab308418--immunocytochemistry-immunofluorescence-img408263.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Strep epitope Tag antibody [HL1251] (ab308418)")
Immunocytochemistry/ Immunofluorescence - Anti-Strep epitope Tag antibody [HL1251] (ab308418)
For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Strep epitope Tag using ab308418 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com