Anti-Strep-tag II antibody [11A7] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Strep tag-GFP transfected 293T cells labelling Strep Tag II with ab252885 at 1/250 dilution (3.808μg/ml), followed by ab150160 Goat Anti-rat IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150160 Goat Anti-rat IgG H&L (Alexa Fluor^® 594) at 1/1000 (2μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885). ab252885 staining Strep-tag II in Strep tag-GFP transfected HEK-293T cells. The cells were fixed with 4% paraformaldehyde and permeabilised in 0.1% Triton X-100. The cells were then incubated with ab252885 at 1/250 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Nucleus were visualized using DAPI. Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector.

• IP

Lab

Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885). Strep Tag II was immunoprecipitated from HEK-293T transfected with GFP expression vector containing a Strep Tag II whole cell lysate with ab252885 at 1/50 dilution. Western blot was performed on the immunoprecipitate using ab252885 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution. Lane 1(Input) : HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate 2 μg Lane 2 (+) : HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate Lane 3 (-) : Rat monoclonal IgG2b (ab18541) instead of ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate Observed MV : 26 kDa Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Strep-tag II antibody [11A7] (<a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-11a7-ab252885'>ab252885</a>) at 1/1000 dilution

Lane 1:

HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate at 2 µg

Lane 2:

HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate

Lane 3:

Rat monoclonal IgG2b (<a href='/en-us/products/primary-antibodies/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-ab18541'>ab18541</a>) instead of <a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-11a7-ab252885'>ab252885</a> in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 26 kDa

false

Exposure time: 5s

• I-ELISA

Lab

Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885). Indirect ELISA analysis of Strep tag II at 1000 ng/ml with ab252885 at 2000-0 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) was used as the secondary antibody at 1/1000 dilution.

• WB

Lab

Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Strep-tag II antibody [11A7] (<a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-11a7-ab252885'>ab252885</a>) at 1/5000 dilution

All lanes:

strep-tagged standard marker at 2 µL

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 1 kDa

false

Exposure time: 1s