Rat Recombinant Monoclonal Strep-tag II antibody. Carrier free. Suitable for IP, WB, I-ELISA, ICC/IF and reacts with Tag samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunocytochemistry-immunofluorescence-img94996.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)")
![Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunoprecipitation-img416138.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)")
![Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--indirect-elisa-img416137.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)")
![Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunocytochemistry-immunofluorescence-img416146.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)")
![Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--western-blot-img416147.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (AB255890)")
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | I-ELISA | ICC/IF | |
|---|---|---|---|---|
| Tag | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Target data
Alternative names
Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II
Recommended products
Rat Recombinant Monoclonal Strep-tag II antibody. Carrier free. Suitable for IP, WB, I-ELISA, ICC/IF and reacts with Tag samples.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 11A7
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255890 is the carrier-free version of Anti-Strep-tag II antibody [11A7] ab252885.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunocytochemistry-immunofluorescence-img94996.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)")
Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Strep tag-GFP transfected 293T cells labelling Strep Tag II with Anti-Strep-tag II antibody [11A7] ab252885 at 1/250 dilution (3.808μg/ml), followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-rat IgG H&L (Alexa Fluor® 594) at 1/1000 (2μg/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Strep-tag II antibody [11A7] ab252885).
![Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunoprecipitation-img416138.jpg "Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)")
Immunoprecipitation - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Strep-tag II antibody [11A7] ab252885).
Strep Tag II was immunoprecipitated from HEK-293T transfected with GFP expression vector containing a Strep Tag II whole cell lysate with Anti-Strep-tag II antibody [11A7] ab252885 at 1/50 dilution. Western blot was performed on the immunoprecipitate using Anti-Strep-tag II antibody [11A7] ab252885 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) was used at 1/10000 dilution.
Lane 1(Input): HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate 2 μg
Lane 2 (+): HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
Lane 3 (-): Rat monoclonal IgG2b (Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control ab18541) instead of Anti-Strep-tag II antibody [11A7] ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
Observed MV: 26 kDa
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-Strep-tag II antibody [11A7] (Anti-Strep-tag II antibody [11A7] ab252885) at 1/1000 dilution
Lane 1: HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate at 2 µg
Lane 2: HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
Lane 3: Rat monoclonal IgG2b (Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control ab18541) instead of Anti-Strep-tag II antibody [11A7] ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
SecondaryAll lanes: Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Observed band size: 26 kDa
Exposure time: 5s
![Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--indirect-elisa-img416137.jpg "Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)")
Indirect ELISA - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Strep-tag II antibody [11A7] ab252885).
Indirect ELISA analysis of Strep tag II at 1000 ng/ml with Anti-Strep-tag II antibody [11A7] ab252885 at 2000-0 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) was used as the secondary antibody at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--immunocytochemistry-immunofluorescence-img416146.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)")
Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Strep-tag II antibody [11A7] ab252885).
Anti-Strep-tag II antibody [11A7] ab252885 staining Strep-tag II in Strep tag-GFP transfected HEK-293T cells. The cells were fixed with 4% paraformaldehyde and permeabilised in 0.1% Triton X-100. The cells were then incubated with Anti-Strep-tag II antibody [11A7] ab252885 at 1/250 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Nucleus were visualized using DAPI.
Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector.![Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890), expandable thumbnail](https://content.abcam.com/products/images/strep-tag-ii-antibody-11a7-bsa-and-azide-free-ab255890--western-blot-img416147.jpg "Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)")
Western blot - Anti-Strep-tag II antibody [11A7] - BSA and Azide free (ab255890)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Strep-tag II antibody [11A7] ab252885).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.All lanes: Western blot - Anti-Strep-tag II antibody [11A7] (Anti-Strep-tag II antibody [11A7] ab252885) at 1/5000 dilution
All lanes: strep-tagged standard marker at 2 µL
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Predicted band size: 1 kDa
Exposure time: 1s
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