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AB76949

Anti-Strep-tag II antibody

4

(7 Reviews)

|

(82 Publications)

Anti-Strep-tag II antibody (ab76949) is a rabbit polyclonal antibody detecting Strep-tag II in Western Blot.

- Over 50 publications
- Trusted since 2009

View Alternative Names

Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II

3 Images
Western blot - Anti-Strep-tag II antibody (AB76949)
  • WB

AbReview34088****

Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1/1000 dilution

All lanes:

Strep-tagged recombinant protein at 0.5 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L)-HRP Conjugate at 1/5000 dilution

Predicted band size: 1 kDa

false

Image courtesy of an anonymous AbReview

Western blot - Anti-Strep-tag II antibody (AB76949)
  • WB

AbReview33348****

Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1/2000 dilution

Lane 1:

Negative control

Lane 2:

cell lysate containing expressed recombinant protein with strep-tag II.

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/1000 dilution

Predicted band size: 1 kDa

false

Image courtesy of an anonymous AbReview

Western blot - Anti-Strep-tag II antibody (AB76949)
  • WB

Unknown

Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1 µg/mL

Lane 1:

StreptII tagged recombinant protein at 0.005 µg

Lane 2:

StrepII tagged recombinant protein at 0.025 µg

Predicted band size: 1 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>Predicted Band Size: 24kDa</p>" } } }

Product details

Anti-Strep-tag II antibody (ab76949) is a rabbit polyclonal antibody and is validated for use in WB in human, mouse, rat, saccharomyces cerevisiae samples.

Anti-Strep-tag II antibody (ab76949) has been cited over 57 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Strep-tag II antibody (ab76949) has high sensitivity and specificity.

Anti-Strep-tag II antibody (ab76949) has 7 independent reviews from customers.

Strep-tag II antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, anti-Strep-tag II antibody (twin Strep tag antibody) (ab76949) has been validated in WB.

Anti-Strep-tag II antibody (ab76949) specifically detects Strep-tag sequence (Strep tag sequence) and is sold in 100 µg selling sizes.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity chromatography purified via peptide column
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (82)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112974 PubMed40687831

2025

Mutation T9I in Envelope confers autophagy resistance to SARS-CoV-2 Omicron.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Klute,Rayhane Nchioua,Arne Cordsmeier,Jyoti Vishwakarma,Lennart Koepke,Hala Alshammary,Christoph Jung,Maximilian Hirschenberger,Helene Hoenigsperger,Jana-Romana Fischer,Rinu Sivarajan,Fabian Zech,Steffen Stenger,Ruth Serra-Moreno,Ana Silvia Gonzalez-Reiche,Emilia Mia Sordillo,Harm van Bakel,Viviana Simon,Frank Kirchhoff,Timo Jacob,Dorota Kmiec,Andreas Pichlmair,Armin Ensser,Konstantin Maria Johannes Sparrer

The EMBO journal 44:3448-3467 PubMed40328966

2025

CHD8 interacts with BCL11A to induce oncogenic transcription in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Waterhouse,Kyren Lazarus,Maria Francesca Santolla,Sara Pensa,Eleanor Williams,Abigail J Q Siu,Hisham Mohammed,Irina Mohorianu,Marcello Maggiolini,Jason Carroll,Laura S Itzhaki,Taufiq Rahman,Walid T Khaled

Nature 642:1090-1096 PubMed40240611

2025

Chromosome end protection by RAP1-mediated inhibition of DNA-PK.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Eickhoff,Ceylan Sonmez,Charlotte E L Fisher,Oviya Inian,Theodoros I Roumeliotis,Angela Dello Stritto,Jörg Mansfeld,Jyoti S Choudhary,Sebastian Guettler,Francisca Lottersberger,Max E Douglas

Science advances 11:eads5143 PubMed40073135

2025

Ndrg3 is a critical regulator of peripheral T cell maturation and homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Komorowska,Christiane Grammer,Mirela Bălan,Jeremy B Swann

PLoS biology 23:e3003030 PubMed40029885

2025

Members of the DIP and Dpr adhesion protein families use cis inhibition to shape neural development in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas C Morano,Davys H Lopez,Hagar Meltzer,Alina P Sergeeva,Phinikoula S Katsamba,Kevin D Rostam,Himanshu Pawankumar Gupta,Jordan E Becker,Bavat Bornstein,Filip Cosmanescu,Oren Schuldiner,Barry Honig,Richard S Mann,Lawrence Shapiro

Nature communications 16:1998 PubMed40011427

2025

Molecular mechanisms of CAND2 in regulating SCF ubiquitin ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Kankan Wang,Lihong Li,Sebastian Kenny,Dailin Gan,Justin M Reitsma,Yun Zhou,Chittaranjan Das,Xing Liu

Molecular cell 85:815-828.e10 PubMed39879985

2025

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Carrillo Roas,Yuichi Yagita,Paul Murphy,Robert Kurzbauer,Tim Clausen,Eszter Zavodszky,Ramanujan S Hegde

Cell chemical biology 32:307-324.e15 PubMed39732052

2024

Discovery of a Pseudomonas aeruginosa-specific small molecule targeting outer membrane protein OprH-LPS interaction by a multiplexed screen.

Applications

Unspecified application

Species

Unspecified reactive species

Bradley E Poulsen,Thulasi Warrier,Sulyman Barkho,Josephine Bagnall,Keith P Romano,Tiantian White,Xiao Yu,Tomohiko Kawate,Phuong H Nguyen,Kyra Raines,Kristina Ferrara,A Lorelei Golas,Michael FitzGerald,Andras Boeszoermenyi,Virendar Kaushik,Michael Serrano-Wu,Noam Shoresh,Deborah T Hung

Nature communications 15:9963 PubMed39551769

2024

Soluble αβ-tubulins reversibly sequester TTC5 to regulate tubulin mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Batiuk,Markus Höpfler,Ana C Almeida,Deryn Teoh En-Jie,Oscar Vadas,Evangelia Vartholomaiou,Ramanujan S Hegde,Zhewang Lin,Ivana Gasic

Scientific reports 14:20697 PubMed39237598

2024

A Gaussia luciferase reporter assay for the evaluation of coronavirus Nsp5/3CLpro activity.

Applications

Unspecified application

Species

Unspecified reactive species

Asimenia Vlachou,Rayhane Nchioua,Kerstin Regensburger,Frank Kirchhoff,Dorota Kmiec
View all publications

Product promise

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