Anti-Strep-tag II antibody (ab76949) is a rabbit polyclonal antibody detecting Strep-tag II in Western Blot.
- Over 50 publications
- Trusted since 2009
View Alternative Names
Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II
- WB
AbReview34088****
Western blot - Anti-Strep-tag II antibody (AB76949)
All lanes:
Western blot - Anti-Strep-tag II antibody (ab76949) at 1/1000 dilution
All lanes:
Strep-tagged recombinant protein at 0.5 µg
Secondary
All lanes:
Goat anti-Rabbit IgG (H+L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 1 kDa
false
Image courtesy of an anonymous AbReview
- WB
AbReview33348****
Western blot - Anti-Strep-tag II antibody (AB76949)
All lanes:
Western blot - Anti-Strep-tag II antibody (ab76949) at 1/2000 dilution
Lane 1:
Negative control
Lane 2:
cell lysate containing expressed recombinant protein with strep-tag II.
Secondary
All lanes:
HRP conjugated goat anti-rabbit at 1/1000 dilution
Predicted band size: 1 kDa
false
Image courtesy of an anonymous AbReview
- WB
Unknown
Western blot - Anti-Strep-tag II antibody (AB76949)
All lanes:
Western blot - Anti-Strep-tag II antibody (ab76949) at 1 µg/mL
Lane 1:
StreptII tagged recombinant protein at 0.005 µg
Lane 2:
StrepII tagged recombinant protein at 0.025 µg
Predicted band size: 1 kDa
false
Reactivity data
Product details
Anti-Strep-tag II antibody (ab76949) has been cited over 57 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Strep-tag II antibody (ab76949) has high sensitivity and specificity.
Anti-Strep-tag II antibody (ab76949) has 7 independent reviews from customers.
Strep-tag II antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, anti-Strep-tag II antibody (twin Strep tag antibody) (ab76949) has been validated in WB.
Anti-Strep-tag II antibody (ab76949) specifically detects Strep-tag sequence (Strep tag sequence) and is sold in 100 µg selling sizes.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (82)
Recent publications for all applications. Explore the full list and refine your search
iScience 28:112974 PubMed40687831
2025
Applications
Unspecified application
Species
Unspecified reactive species
The EMBO journal 44:3448-3467 PubMed40328966
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature 642:1090-1096 PubMed40240611
2025
Applications
Unspecified application
Species
Unspecified reactive species
Science advances 11:eads5143 PubMed40073135
2025
Applications
Unspecified application
Species
Unspecified reactive species
PLoS biology 23:e3003030 PubMed40029885
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:1998 PubMed40011427
2025
Applications
Unspecified application
Species
Unspecified reactive species
Molecular cell 85:815-828.e10 PubMed39879985
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell chemical biology 32:307-324.e15 PubMed39732052
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 15:9963 PubMed39551769
2024
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 14:20697 PubMed39237598
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com