AB76949

Anti-Strep-tag II antibody

Name: Anti-strep-tag II antibody - rabbit polyclonal (ab76949) | Abcam
Brand: Abcam Limited
SKU: AB76949
Price: 400 USD
Availability: InStock
Rating: 4 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(57 Publications)

Anti-Strep-tag II antibody (ab76949) is a rabbit polyclonal antibody detecting Strep-tag II in Western Blot.

- Over 50 publications
- Trusted since 2009

View Alternative Names

Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II

3 Images

AbReview34088****

Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1/1000 dilution

All lanes:

Strep-tagged recombinant protein at 0.5 µg with 5% milk in TBS-T

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L)-HRP Conjugate at 1/5000 dilution

Predicted band size: 1 kDa

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Image courtesy of an anonymous AbReview

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Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1 µg/mL

Lane 1:

StreptII tagged recombinant protein at 0.005 µg

Lane 2:

StrepII tagged recombinant protein at 0.025 µg

Predicted band size: 1 kDa

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AbReview33348****

Western blot - Anti-Strep-tag II antibody (AB76949)

All lanes:

Western blot - Anti-Strep-tag II antibody (ab76949) at 1/2000 dilution

Lane 1:

Negative control with PBS with 0.5% milk and 0.1% Tween-20

Lane 2:

cell lysate containing expressed recombinant protein with strep-tag II. with PBS with 0.5% milk and 0.1% Tween-20

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/1000 dilution

Predicted band size: 1 kDa

false

Image courtesy of an anonymous AbReview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>Predicted Band Size: 24kDa</p>" } } }

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Product details

Anti-Strep-tag II antibody (ab76949) is a rabbit polyclonal antibody and is validated for use in WB in human, mouse, rat, saccharomyces cerevisiae samples.

Anti-Strep-tag II antibody (ab76949) has been cited over 57 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Strep-tag II antibody (ab76949) has high sensitivity and specificity.

Anti-Strep-tag II antibody (ab76949) has 7 independent reviews from customers.

Strep-tag II antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, anti-Strep-tag II antibody (twin Strep tag antibody) (ab76949) has been validated in WB.

Anti-Strep-tag II antibody (ab76949) specifically detects Strep-tag sequence (Strep tag sequence) and is sold in 100 µg selling sizes.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity chromatography purified via peptide column

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Strep-tag II

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:228 PubMed36990977

2023

Influenza viral matrix 1 protein aggravates viral pathogenicity by inducing TLR4-mediated reactive oxygen species production and apoptotic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Ung Kim,Dahwan Lim,Young Sang Kim,Bonsu Ku,Doo-Jin Kim

PloS one 18:e0280336 PubMed36634117

2023

PlrA (MSMEG_5223) is an essential polar growth regulator in Mycobacterium smegmatis.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Y Quintanilla,Neda Habibi Arejan,Parthvi B Patel,Cara C Boutte

Cells 11: PubMed36552858

2022

TRIP13 Participates in Immediate-Early Sensing of DNA Strand Breaks and ATM Signaling Amplification through MRE11.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeongsun Jeong,Minwoo Wie,In-Joon Baek,Gyuwon Sohn,Si-Hyeon Um,Seon-Gyeong Lee,Yuri Seo,Jaesun Ra,Eun A Lee,Shinseog Kim,Byung Gyu Kim,Rajashree A Deshpande,Tanya T Paull,Joo Seok Han,Taejoon Kwon,Kyungjae Myung

Molecular microbiology 119:208-223 PubMed36416406

2022

Arginine methylation sites on SepIVA help balance elongation and septation in Mycobacterium smegmatis.

Applications

Unspecified application

Species

Unspecified reactive species

Angela H Freeman,Karen Tembiwa,James R Brenner,Michael R Chase,Sarah M Fortune,Yasu S Morita,Cara C Boutte

International journal of molecular sciences 23: PubMed36430190

2022

A Vaccine of SARS-CoV-2 S Protein RBD Induces Protective Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoqiao Qu,Pengfei Hao,Wang Xu,Letian Li,Yuhang Jiang,Zhiqiang Xu,Jing Chen,Zihan Gao,Zhaoxia Pang,Ningyi Jin,Chang Li

Nature communications 13:4434 PubMed35907924

2022

Structural basis for assembly and disassembly of the IGF/IGFBP/ALS ternary complex.

Applications

Unspecified application

Species

Unspecified reactive species

Hyojin Kim,Yaoyao Fu,Ho Jeong Hong,Seong-Gyu Lee,Dong Sun Lee,Ho Min Kim

The EMBO journal 41:e107896 PubMed35811551

2022

Mitotic checkpoint gene expression is tuned by codon usage bias.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Esposito,Douglas E Weidemann,Jessie M Rogers,Claire M Morton,Erod Keaton Baybay,Jing Chen,Silke Hauf

Developmental cell 57:1712-1727.e9 PubMed35809563

2022

A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.

Applications

Unspecified application

Species

Unspecified reactive species

Dilyana Georgieva Kirova,Kristyna Judasova,Julia Vorhauser,Thomas Zerjatke,Jacky Kieran Leung,Ingmar Glauche,Jörg Mansfeld

Molecular microbiology 118:47-60 PubMed35670057

2022

Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Farah Shamma,E Hesper Rego,Cara C Boutte

Bio-protocol 12:e4421 PubMed35813025

2022

Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Hendry Susila,Snježana Juric,Katarzyna Gawarecka,Kyung Sook Chung,Suhyun Jin,Soo-Jin Kim,Zeeshan Nasim,Geummin Youn,Ji Hoon Ahn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Strep-tag II antibody

Western blot - Anti-Strep-tag II antibody (AB76949)

Western blot - Anti-Strep-tag II antibody (AB76949)

Western blot - Anti-Strep-tag II antibody (AB76949)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (57)

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