AB232586

Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Strep-tag II antibody. Carrier free. Suitable for WB and reacts with Tag samples. Cited in 3 publications.

View Alternative Names

Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II

1 Images

Supplier Data

Western blot - Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free (AB232586)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180957).

All lanes:

Western blot - Anti-Strep-tag II antibody [EPR12666] (<a href='/en-us/products/primary-antibodies/strep-tag-ii-antibody-epr12666-ab180957'>ab180957</a>) at 1/1000 dilution

Lane 1:

Strep-tag II protein at 0.01 µg

Lane 2:

Strep-tag II protein at 0.005 µg

Lane 3:

Strep-tag II protein at 0.001 µg

Lane 4:

Strep-tag II protein at 0.0005 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 1 kDa

false

Unconjugated

Anti-Strep-tag II antibody [EPR12666]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12666

Isotype

IgG

Carrier free

Yes

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

ab232586 is the carrier-free version of ab180957.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Strep-tag II

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 602:487-495 PubMed34942634

2021

Evolution of enhanced innate immune evasion by SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy G Thorne,Mehdi Bouhaddou,Ann-Kathrin Reuschl,Lorena Zuliani-Alvarez,Ben Polacco,Adrian Pelin,Jyoti Batra,Matthew V X Whelan,Myra Hosmillo,Andrea Fossati,Roberta Ragazzini,Irwin Jungreis,Manisha Ummadi,Ajda Rojc,Jane Turner,Marie L Bischof,Kirsten Obernier,Hannes Braberg,Margaret Soucheray,Alicia Richards,Kuei-Ho Chen,Bhavya Harjai,Danish Memon,Joseph Hiatt,Romel Rosales,Briana L McGovern,Aminu Jahun,Jacqueline M Fabius,Kris White,Ian G Goodfellow,Yasu Takeuchi,Paola Bonfanti,Kevan Shokat,Natalia Jura,Klim Verba,Mahdad Noursadeghi,Pedro Beltrao,Manolis Kellis,Danielle L Swaney,Adolfo García-Sastre,Clare Jolly,Greg J Towers,Nevan J Krogan

bioRxiv : the preprint server for biology : PubMed34127972

2021

Evolution of enhanced innate immune evasion by the SARS-CoV-2 B.1.1.7 UK variant.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy G Thorne,Mehdi Bouhaddou,Ann-Kathrin Reuschl,Lorena Zuliani-Alvarez,Ben Polacco,Adrian Pelin,Jyoti Batra,Matthew V X Whelan,Manisha Ummadi,Ajda Rojc,Jane Turner,Kirsten Obernier,Hannes Braberg,Margaret Soucheray,Alicia Richards,Kuei-Ho Chen,Bhavya Harjai,Danish Memon,Myra Hosmillo,Joseph Hiatt,Aminu Jahun,Ian G Goodfellow,Jacqueline M Fabius,Kevan Shokat,Natalia Jura,Klim Verba,Mahdad Noursadeghi,Pedro Beltrao,Danielle L Swaney,Adolfo Garcia-Sastre,Clare Jolly,Greg J Towers,Nevan J Krogan

Science (New York, N.Y.) 370: PubMed33060197

2020

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

David E Gordon,Joseph Hiatt,Mehdi Bouhaddou,Veronica V Rezelj,Svenja Ulferts,Hannes Braberg,Alexander S Jureka,Kirsten Obernier,Jeffrey Z Guo,Jyoti Batra,Robyn M Kaake,Andrew R Weckstein,Tristan W Owens,Meghna Gupta,Sergei Pourmal,Erron W Titus,Merve Cakir,Margaret Soucheray,Michael McGregor,Zeynep Cakir,Gwendolyn Jang,Matthew J O'Meara,Tia A Tummino,Ziyang Zhang,Helene Foussard,Ajda Rojc,Yuan Zhou,Dmitry Kuchenov,Ruth Hüttenhain,Jiewei Xu,Manon Eckhardt,Danielle L Swaney,Jacqueline M Fabius,Manisha Ummadi,Beril Tutuncuoglu,Ujjwal Rathore,Maya Modak,Paige Haas,Kelsey M Haas,Zun Zar Chi Naing,Ernst H Pulido,Ying Shi,Inigo Barrio-Hernandez,Danish Memon,Eirini Petsalaki,Alistair Dunham,Miguel Correa Marrero,David Burke,Cassandra Koh,Thomas Vallet,Jesus A Silvas,Caleigh M Azumaya,Christian Billesbølle,Axel F Brilot,Melody G Campbell,Amy Diallo,Miles Sasha Dickinson,Devan Diwanji,Nadia Herrera,Nick Hoppe,Huong T Kratochvil,Yanxin Liu,Gregory E Merz,Michelle Moritz,Henry C Nguyen,Carlos Nowotny,Cristina Puchades,Alexandrea N Rizo,Ursula Schulze-Gahmen,Amber M Smith,Ming Sun,Iris D Young,Jianhua Zhao,Daniel Asarnow,Justin Biel,Alisa Bowen,Julian R Braxton,Jen Chen,Cynthia M Chio,Un Seng Chio,Ishan Deshpande,Loan Doan,Bryan Faust,Sebastian Flores,Mingliang Jin,Kate Kim,Victor L Lam,Fei Li,Junrui Li,Yen-Li Li,Yang Li,Xi Liu,Megan Lo,Kyle E Lopez,Arthur A Melo,Frank R Moss,Phuong Nguyen,Joana Paulino,Komal Ishwar Pawar,Jessica K Peters,Thomas H Pospiech,Maliheh Safari,Smriti Sangwan,Kaitlin Schaefer,Paul V Thomas,Aye C Thwin,Raphael Trenker,Eric Tse,Tsz Kin Martin Tsui,Feng Wang,Natalie Whitis,Zanlin Yu,Kaihua Zhang,Yang Zhang,Fengbo Zhou,Daniel Saltzberg,Anthony J Hodder,Amber S Shun-Shion,Daniel M Williams,Kris M White,Romel Rosales,Thomas Kehrer,Lisa Miorin,Elena Moreno,Arvind H Patel,Suzannah Rihn,Mir M Khalid,Albert Vallejo-Gracia,Parinaz Fozouni,Camille R Simoneau,Theodore L Roth,David Wu,Mohd Anisul Karim,Maya Ghoussaini,Ian Dunham,Francesco Berardi,Sebastian Weigang,Maxime Chazal,Jisoo Park,James Logue,Marisa McGrath,Stuart Weston,Robert Haupt,C James Hastie,Matthew Elliott,Fiona Brown,Kerry A Burness,Elaine Reid,Mark Dorward,Clare Johnson,Stuart G Wilkinson,Anna Geyer,Daniel M Giesel,Carla Baillie,Samantha Raggett,Hannah Leech,Rachel Toth,Nicola Goodman,Kathleen C Keough,Abigail L Lind,Reyna J Klesh,Kafi R Hemphill,Jared Carlson-Stevermer,Jennifer Oki,Kevin Holden,Travis Maures,Katherine S Pollard,Andrej Sali,David A Agard,Yifan Cheng,James S Fraser,Adam Frost,Natalia Jura,Tanja Kortemme,Aashish Manglik,Daniel R Southworth,Robert M Stroud,Dario R Alessi,Paul Davies,Matthew B Frieman,Trey Ideker,Carmen Abate,Nolwenn Jouvenet,Georg Kochs,Brian Shoichet,Melanie Ott,Massimo Palmarini,Kevan M Shokat,Adolfo García-Sastre,Jeremy A Rassen,Robert Grosse,Oren S Rosenberg,Kliment A Verba,Christopher F Basler,Marco Vignuzzi,Andrew A Peden,Pedro Beltrao,Nevan J Krogan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free

Western blot - Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free (AB232586)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise