Anti-Strep-tag II antibody [EPR28119-43] 20 ul size (ab307676)

Anti-Strep-tag II antibody [EPR28119-43] ab307676 is a rabbit monoclonal antibody that is used in Strep-tag II western blotting, IHC, immunofluorescence and flow cytometry.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Strep-tag II staining

Be the first to review this product! Submit a review

Images

Western blot - Anti-Strep-tag II antibody [EPR28119-43] (AB307676), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IP	ELISA
Tag	Tested	Tested	Tested	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Tag	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Tag	Dilution info 0.125 µg/mL	Notes -

Target data

See full target information Strep-tag II

Alternative names

Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantiboy, anti-Strep-Tag II

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28119-43
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Buffer and diluting concentration: 5% NFDM/TBST
Primary dilution of 1/1000 and a secondary dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051), of 1/100,000
Exposure time: 1 second
Lane 1: Western blot - Anti-Strep-tag II antibody [EPR28119-43] (ab307676) at 1/1000 dilution
Lane 1: Western blot - Anti-Strep-tag II antibody [EPR28119-43] - BSA and Azide free (Anti-Strep-tag II antibody [EPR28119-43] - BSA and Azide free ab307677)
All lanes: Strep-tagged standard marker (mixture of Strep-tagged recombinant proteins,10-250 kDa)
Secondary
All lanes: Western blot at 1/100000 dilution
Observed band size: 10 kDa, 15 kDa, 20 kDa, 25 kDa, 37 kDa, 50 kDa, 75 kDa, 100 kDa, 150 kDa, 250 kDa
Exposure time: 1s
Flow Cytometry (Intracellular) - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with a Strep-tag II expression vector containing a GFP tag cells labelling Strep-tag II with ab307676 at 1/5000 dilution (0.01ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Strep-tag II was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Strep-tag II expression vector containing a GFP tag whole cell lysate 10 ug with ab307676 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307676 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Strep-tag II expression vector containing a GFP tag whole cell lysate 10 ug

Lane 2: abab307676 IP in 293T cells transfected with a Strep-tag II expression vector containing a GFP tag whole cell lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307676 in 293Tcells transfected with a Strep-tag II expression vector containing a GFP tag whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds
Observed band size: 30 kDa
Exposure time: 3s
Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling Strep-tag II with ab307676 at 1/100 (5.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preabsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a Strep-tag II expression vector containing a myc-tag. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preabsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Strep-tag II with ab307676 at 1/5000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on rat colon. The section was incubated with ab307676 for 30 mins at room temperature .The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Strep-tag II with ab307676 at 1/5000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on mouse colon. The section was incubated with ab307676 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Strep-tag II with ab307676 at 1/5000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human colon. The section was incubated with ab307676 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Strep-tag II with ab307676 at 1/5000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on (A) HEK-293T cells transfected with a gfp-strep II (WSHPQFEK) expression vector containing a myc-tag. No staining on (B) HEK-293T cells transfected with empty vector containing a myc-tag. The section was incubated with ab307676 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Strep-tag II antibody [EPR28119-43] (ab307676)
Buffer and diluting concentration: 5% NFDM/TBST

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

Primary dilution of 1/1000 and a secondary dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051), of 1/100,000

Exposure time: 3 seconds
All lanes: Western blot - Anti-Strep-tag II antibody [EPR28119-43] (ab307676) at 1/1000 dilution
Lane 1: 293T cells transfected with a human Strep-tag II expression vector containing a GFP tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with an empty vector containing a GFP tag whole cell lysate at 20 µg
Secondary
All lanes: Western blot at 1/100000 dilution
Observed band size: 30 kDa
Exposure time: 3s