AB184224

Anti-Strep-tag II antibody [GT661]

Lab Essentials
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(18 Publications)

Anti-Strep-tag II antibody [GT661] (ab184224) is a mouse monoclonal antibody detecting Strep-tag II in Western Blot, IP, ICC/IF.

- Over 10 publications

3 Images

Supplier Data

Western blot - Anti-Strep-tag II antibody [GT661] (AB184224)

12 % SDS-PAGE

All lanes:

Western blot - Anti-Strep-tag II antibody [GT661] (ab184224) at 1/5000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

whole cell lysate of strep-tagged II protein expressing 293T cells at 30 µg

Predicted band size: 1 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (AB184224)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (AB184224)

Immunofluorescent analysis of Strep II/GFP-tag transfected HeLa cells (fixed in 4% paraformaldehyde at RT for 15 min) showing labeling of Strep-recombinant protein by ab184224 used at 1/500 dilution. Red : Strep-recombinant protein stained by Strep-tag antibody (ab184224) diluted at 1 : 500. Green : GFP is expressed in the tranfected cell. Blue : Hoechst 33342 staining.

Supplier Data

Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (AB184224)

ab184224 immunoprecipitates strep tagged protein in IP experiments. IP Sample : 293T whole cell lysate/extract.

Lane 1 : 20 μg whole cell lysate/extract of strep II tagged protein expressing 293T cells

Lane 2 : Control with 2.5 μg of pre-immune mouse IgG

Lane 3 : Immunoprecipitation of strep tagged protein by 2.5 μg of strep tag antibody (ab184224).

10% SDS-PAGE -The immunoprecipitated strep tagged protein was detected by strep tag antibody (ab184224) diluted at 1/3000. Anti-mouse IgG (HRP) antibody was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)

Predicted band size: 1 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT661

Isotype

IgG2a

Carrier free

Applications

WB, ICC/IF, IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/3000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Strep-tag II antibody [GT661] (ab184224) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
Strep-tag II antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-Strep-tag II [GT661] (ab184224) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Strep-tag II

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115728 PubMed40381193

2025

An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary Walter,Minghua Li,Melissa Molho,Lauren Berish,Andrew Isopi,Mary O'Mara,Mark Dittmar,Chike Nwaezeapu,Alicia Richards,Martin McCullagh,Nevan J Krogan,Sara Cherry,Jeffrey R Johnson,Holly Ramage

Stem cell research & therapy 16:230 PubMed40336034

2025

Human menstrual blood-derived stem cells secreted ECM1 directly interacts with LRP1α to ameliorate hepatic fibrosis through FoxO1 and mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yangxin Fang,Lin Chen,Yin Yuan,Sining Zhou,Jiamin Fu,Qi Zhang,Ning Zhang,Yuqi Huang,Yifei Li,Li Yuan,Lijun Chen,Charlie Xiang

Communications biology 7:1089 PubMed39237833

2024

Inhibition of sterol O-acyltransferase 1 blocks Zika virus infection in cell lines and cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Schöbel,Vinicius Pinho Dos Reis,Rabea Burkhard,Julia Hehner,Laura Schneider,Martin Schauflinger,Gabrielle Vieyres,Eva Herker

Nature communications 15:3584 PubMed38678026

2024

Mechanism of DNA unwinding by MCM8-9 in complex with HROB.

Applications

Unspecified application

Species

Unspecified reactive species

Ananya Acharya,Hélène Bret,Jen-Wei Huang,Martin Mütze,Martin Göse,Vera Maria Kissling,Ralf Seidel,Alberto Ciccia,Raphaël Guérois,Petr Cejka

Journal of virology 98:e0190923 PubMed38289118

2024

SARS-CoV-2 and oncolytic EV-D68-encoded proteases differentially regulate pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Shen,Haoran Guo,Yan Li,Lili Zhang,Yubin Tang,Huili Li,Xiaohan Li,Pei-Hui Wang,Xiao-Fang Yu,Wei Wei

The EMBO journal 42:e112542 PubMed37218505

2023

Coronaviral ORF6 protein mediates inter-organelle contacts and modulates host cell lipid flux for virus production.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhen Yue,Bing Hu,Jiajia Li,Ruifeng Chen,Zhen Yuan,Hurong Xiao,Haishuang Chang,Yaming Jiu,Kun Cai,Binbin Ding

Cell reports 42:112286 PubMed36952345

2023

Coronavirus subverts ER-phagy by hijacking FAM134B and ATL3 into p62 condensates to facilitate viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Tan,Kun Cai,Jiajia Li,Zhen Yuan,Ruifeng Chen,Hurong Xiao,Chuanrui Xu,Bing Hu,Yali Qin,Binbin Ding

International journal of molecular sciences 24: PubMed36768595

2023

Homologs of Ancestral CNNM Proteins Affect Magnesium Homeostasis and Circadian Rhythmicity in a Model Eukaryotic Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio Gil,Helen K Feord,Gerben van Ooijen

Biomedicines 9: PubMed34944610

2021

COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Sansico,Mattia Miroballo,Daniele Salvatore Bianco,Francesco Tamiro,Mattia Colucci,Elisabetta De Santis,Giovanni Rossi,Jessica Rosati,Lazzaro Di Mauro,Giuseppe Miscio,Tommaso Mazza,Angelo Luigi Vescovi,Gianluigi Mazzoccoli,Vincenzo Giambra, On Behalf Of Css-Covid Group

Frontiers in microbiology 12:615165 PubMed33717005

2021

The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lien De Caluwé,Sandra Coppens,Katleen Vereecken,Simon Daled,Maarten Dhaenens,Xaveer Van Ostade,Dieter Deforce,Kevin K Ariën,Koen Bartholomeeusen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Strep-tag II antibody [GT661]

Western blot - Anti-Strep-tag II antibody [GT661] (AB184224)

Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (AB184224)

Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (AB184224)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (18)

Product promise