Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11]

9 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat cerebrum. The section was incubated with ab316006 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse cerebrum.The section was incubated with ab316006 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on human cerebrum. The section was incubated with ab316006 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®. (B) HEK-293T transfected with empty vector containing a myc-His-tag®. Tissue labeling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on HEK-293T transfected with a Myc-His-tagged Streptococcus mutans Cell surface antigen I/II construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab316006 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Western blot - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 4 µg with 5% NFDM/TBST

  Lane 2: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate at 4 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 260 kDa, 36 kDa

  Exposure time: 3s

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  Immunocytochemistry/ Immunofluorescence - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag® cells labelling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/250 (2.024 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

  Confocal image showing positive staining in 293T transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

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  Immunoprecipitation - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Streptococcus mutans Cell surface antigen I/II was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate with ab316006 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316006 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate
  
  Lane 2: ab316006 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316006 in 293T cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate

  All lanes: Immunoprecipitation - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006) at 1/30 dilution

  All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag®, whole cell lysate with 5% NFDM/TBST

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 6s

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  Indirect ELISA - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Indirect ELISA analysis of ab316006 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

  Antigen: Streptococcus mutans Cell surface antigen I/II.

  Antigen concentration: 1000 ng/ml

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  Flow Cytometry (Intracellular) - Anti-Streptococcus mutans Cell surface antigen I/II antibody [EPR28235-11] (ab316006)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T transfected with a Streptococcus mutans Cell surface antigen I/II expression vector containing a myc-His-tag® (Middle) / 293T transfected with an empty expression vector (Right) cells labelling Streptococcus mutans Cell surface antigen I/II with ab316006 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  Cells were surface stained with primary antibody. Fixed with 2% PFA for 10 min followed by intracellularly staining with anti-Myc tag conjugated to Alexa Fluor® 647.