Rabbit Recombinant Monoclonal Streptococcus mutans Glucan-binding protein antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Streptococcus mutans, Transfected cell line - Streptococcus mutans, Recombinant fragment - Streptococcus mutans samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | I-ELISA | |
---|---|---|---|---|---|---|
Recombinant fragment - Streptococcus mutans | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Streptococcus | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
Transfected cell line - Streptococcus mutans | Not recommended | Tested | Tested | Tested | Not recommended | Not recommended |
Transfected cell lysate - Streptococcus mutans | Tested | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Streptococcus mutans, Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Streptococcus mutans | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans, Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans, Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans, Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Streptococcus mutans, Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Streptococcus mutans | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Streptococcus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Streptococcus mutans, Transfected cell line - Streptococcus mutans | Dilution info - | Notes - |
Select an associated product type
Rabbit Recombinant Monoclonal Streptococcus mutans Glucan-binding protein antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Streptococcus mutans, Transfected cell line - Streptococcus mutans, Recombinant fragment - Streptococcus mutans samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR28235-85
Affinity purification Protein A
This antibody detects transfected levels of Streptococcus mutans Glucan-binding protein.
Blue Ice
+4°C
ab315976 is the carrier-free version of Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cerebrum.The section was incubated with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cerebrum.The section was incubated with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.The section was incubated with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®. (B) HEK-293T transfected with empty vector containing a myc-His-tag®. tissue labeling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on HEK-293T transfected with a Myc-His-tagged Streptococcus mutans Glucan-binding protein construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] (Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 4 µg
Lane 2: 293T cells transfected with a Streptococcus mutans Glucan-bindi protein expression vector containing a myc-His-tag®, whole cell lysate at 4 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 78 kDa, 36 kDa
Exposure time: 8s
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag® cells labelling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/250 (2.024 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining in 293T transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain at 1/100 (0.38ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Streptococcus mutans Glucan-binding protein was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 in 293T cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
All lanes: Immunoprecipitation - Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] (Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
Streptococcus mutans Glucan-binding protein was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 IP in 293T (human embryonic kidney epithelial cell) cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 in 293T cells transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag®, whole cell lysate
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Streptococcus mutans Glucan-binding protein.
Antigen concentration: 1000 ng/ml
This data was developed using Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T transfected with a Streptococcus mutans Glucan-binding protein expression vector containing a myc-His-tag® (Middle) / 293T transfected with an empty expression vector (Right) cells labelling Streptococcus mutans Glucan-binding protein with Anti-Streptococcus mutans Glucan-binding protein antibody [EPR28235-85] ab315975 at 1/500 dilution (0.1 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells are co-stained with Myc tag conjugated to Alexa Fluor®488.
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